Recombinant Human PCDHb3 protein

**Background of PCDHb3 Recombinant Protein**

Protocadherin beta-3 (PCDHb3) is a member of the protocadherin β gene cluster, a subgroup within the cadherin superfamily of calcium-dependent cell adhesion molecules. These transmembrane proteins play critical roles in neural development, including axon guidance, synaptic specificity, and neuronal circuit formation. The protocadherin β cluster, located on human chromosome 5. consists of 16 genes (PCDHb1 to PCDHb16) that encode diverse isoforms. PCDHb3 is particularly notable for its involvement in establishing cell-cell recognition and adhesion through homophilic interactions, which are essential for maintaining neural connectivity and tissue architecture.

Recombinant PCDHb3 protein is engineered *in vitro* by expressing the PCDHb3 gene in heterologous systems, such as mammalian cells (e.g., HEK293) or bacterial systems, followed by purification. This protein typically retains key functional domains, including extracellular cadherin repeats and a conserved cytoplasmic domain, enabling studies on its structural and mechanistic properties. Researchers utilize recombinant PCDHb3 to investigate its role in neurodevelopment, synaptic plasticity, and potential links to neurological disorders. For instance, dysregulation of protocadherins has been implicated in autism spectrum disorders (ASD), schizophrenia, and epilepsy, making PCDHb3 a target for exploring disease mechanisms.

Furthermore, recombinant PCDHb3 serves as a tool for developing antibodies, mapping interaction partners, and screening therapeutic compounds. Its applications extend to *in vitro* assays, such as cell adhesion or neurite outgrowth experiments, and *in vivo* models to validate functional pathways. Recent studies also highlight its involvement in cancer progression, particularly in metastasis and cell signaling, broadening its relevance beyond neuroscience. By providing a controlled, high-purity form of the protein, recombinant PCDHb3 accelerates both basic research and translational efforts to uncover its biological significance and therapeutic potential.