Recombinant Human RPLP2 protein

中文名： 核糖体蛋白大P2(RPLP2)重组蛋白
别名： RPLP2；D11S2243E；RPP2；Large ribosomal subunit protein P2

货号: PA2000-1157

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	RPLP2
Uniprot No	P05387
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-115aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSHMRYVAS YLLAALGGNS SPSAKDIKKI LDSVGIEADD DRLNKVISEL NGKNIEDVIA QGIGKLASVP AGGAVAVSAA PGSAAPAAGS APAAAEEKKD EKKEESEESD DDMGFGLFD
预测分子量	14 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于RPLP2重组蛋白的3篇参考文献(示例格式，部分内容基于真实研究总结)：

1. **文献名称**: "Structural and functional characterization of the human ribosomal protein RPLP2"

**作者**: Martínez-Azorín F, et al.

**摘要**: 通过重组表达纯化人源RPLP2蛋白，解析其与核糖体大亚基的相互作用，揭示了RPLP2在蛋白质合成中的结构功能，并发现其C端酸性区域对核糖体稳定性至关重要。

2. **文献名称**: "RPLP2 promotes colorectal cancer cell proliferation and invasion via MAPK signaling pathway"

**作者**: Li Y, et al.

**摘要**: 利用重组RPLP2蛋白进行体外实验，发现其过表达通过激活MAPK通路促进结直肠癌细胞增殖和迁移，提示RPLP2可能作为癌症治疗靶点。

3. **文献名称**: "Recombinant RPLP2 as a potential biomarker for autoimmune diseases"

**作者**: García-González M, et al.

**摘要**: 研究发现重组RPLP2蛋白在系统性红斑狼疮患者血清中与自身抗体显著结合，提示其可能作为自身免疫疾病的诊断标志物。

(注：以上文献为示例，实际引用时请通过PubMed/Google Scholar检索真实文献。)

背景信息

**Background of RPLP2 Recombinant Protein**

The ribosomal protein lateral stalk subunit P2 (RPLP2) is a component of the 60S ribosomal subunit, playing a critical role in protein synthesis. It belongs to the P-protein family, which includes P0. P1. and P2 subunits. These proteins form the ribosomal "stalk," a flexible structure essential for recruiting translation elongation factors (e.g., eEF-2) during the elongation phase of translation. RPLP2 is evolutionarily conserved, underscoring its fundamental role in ribosome function across species.

Recombinant RPLP2 refers to the protein produced artificially via genetic engineering, typically using expression systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of highly pure, functional RPLP2 for research and therapeutic applications. Its recombinant form often includes tags (e.g., His-tag) for simplified purification and detection.

RPLP2 has garnered attention in biomedical research due to its dual role in cellular homeostasis and disease. Beyond its ribosomal function, RPLP2 is implicated in extraribosomal activities, including cell proliferation, apoptosis, and immune regulation. Dysregulation of RPLP2 expression has been linked to cancers, autoimmune disorders, and viral infections. For example, elevated RPLP2 levels are observed in colorectal and breast cancers, suggesting its potential as a diagnostic or prognostic biomarker.

In autoimmune diseases like systemic lupus erythematosus (SLE), RPLP2 serves as an autoantigen, driving pathogenic antibody responses. Studies using recombinant RPLP2 have helped elucidate its antigenic properties and interactions with the immune system.

Additionally, recombinant RPLP2 is a valuable tool for structural biology, enabling detailed analysis of ribosome assembly and translation mechanisms. Its application in drug discovery includes targeting ribosome biogenesis in cancer or infectious diseases.

Overall, RPLP2 recombinant protein bridges basic ribosome biology and translational medicine, offering insights into both cellular processes and therapeutic strategies.