Recombinant Human GBP7 protein

中文名： 鸟苷酸结合蛋白7(GBP7)重组蛋白
别名： GBP7；GBP4L；Guanylate-binding protein 7

货号: PA2000-919DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	GBP 7
Uniprot No	Q8N8V2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-638aa
氨基酸序列	MASEIHMPGP VCLTENTKGH LVVNSEALEI LSAITQPVVV VAIVGLYRTG KSYLMNKLAG KNKGFPLGCT VKSETKGIWM WCVPHPSKPN HTLILLDTEG LGDMEKSDPK SDSWIFALAV LLSSSFVYNS MGTINHQALE QLHYVTELTE LIRAKSCPRP DEVEDSSEFV SFFPDFIWTV RDFTLELKLD GHPITEDEYL ENALKLISGK NPQIQNSNKP REWIRHFFPK QKCFVFDRPI NDKKLLLHVE EVREDQLDSN FQMQSENFCS YIFTHAKTKT LREGILVTGN RLGMLVETYL DAINSGATPC LENAMAVLAQ CENSAAVQRA ANHYSQQMAQ QVRFPTDTLQ ELLDVHAVCE REAIAVFMEY SFKDKSQEFQ KKLVDTMEKK KEDFVLQNEE ASAKYCQAEL KRLSELLTES ISRGTFFVPG GHNIYLEAKK KIEQDYTLVP RKGVKADEVL QSFLQSQVVI EESILQSDKA LTAGEKAIAA KQAKKEAAEK EQELLRQKQK EQQQMMEAQE RSFQENIAQL KKKMEREREN YMRELRKMLS HKMKVLEELL TEGFKEIFES LNEEINRLKE QIEAAENEEP SVFSQILDVA GSIFIAALPG AAKLVDLGMK ILSSLCNRLR NPGKKIIS
预测分子量	72,5 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于GBP7重组蛋白的参考文献及简要摘要：

1. **文献名称**: *"Recombinant expression and biochemical characterization of human guanylate-binding protein 7 (GBP7)"*

**作者**: Müller, M.P. et al.

**摘要**: 本研究利用大肠杆菌系统成功表达并纯化重组人源GBP7蛋白，通过酶活性和结构分析揭示其核苷酸结合特性及水解活性，为研究其在先天免疫中的作用提供基础。

2. **文献名称**: *"Structural insights into GTP-induced conformational changes of GBP7 and its role in pathogen defense"*

**作者**: Kim, Y. & Lee, S.

**摘要**: 通过X射线晶体学解析GTP结合状态下重组GBP7的构象变化，发现其寡聚化能力与抑制胞内病原体(如沙门氏菌)增殖的直接关联。

3. **文献名称**: *"GBP7 interacts with autophagy receptors to promote antimicrobial defense via recombinant protein assays"*

**作者**: Chen, L. et al.

**摘要**: 利用重组GBP7蛋白的体外结合实验，证明其通过与LC3等自噬相关蛋白相互作用，调控选择性自噬通路以清除胞内病原体。

4. **文献名称**: *"Functional divergence of GBP7 in inflammasome activation revealed by recombinant mutant analysis"*

**作者**: Rossi, F. et al.

**摘要**: 通过构建重组GBP7突变体，发现其特定结构域对NLRP3炎症小体激活的关键作用，为炎症性疾病治疗提供潜在靶点。

注：上述文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“GBP7 recombinant”“Guanylate-binding protein 7 expression”检索最新文献。

背景信息

**Background of GBP7 Recombinant Protein**

Guanylate-binding protein 7 (GBP7) is a member of the guanylate-binding protein (GBP) family, a group of interferon (IFN)-inducible GTPases known for their roles in innate immunity and host defense. GBPs are evolutionarily conserved in vertebrates and play critical functions in cell-autonomous immunity against bacterial, viral, and parasitic pathogens. GBP7. like other GBPs, is strongly induced by proinflammatory cytokines, particularly IFN-γ, and is implicated in mediating immune responses through pathogen recognition, membrane disruption, and coordination of downstream inflammatory signaling.

Structurally, GBP7 contains a globular GTPase domain and unique C-terminal motifs that enable oligomerization and interactions with membranes or intracellular pathogens. It localizes to the cytosol but can translocate to pathogen-containing vacuoles or cellular compartments to disrupt microbial replication. Studies suggest GBP7 contributes to inflammasome activation, cytokine production, and autophagy, enhancing the clearance of intracellular pathogens such as *Salmonella* and *Toxoplasma gondii*. Its ability to sense membrane integrity or pathogen-associated patterns underscores its role as a cellular surveillance protein.

Recombinant GBP7 protein is engineered for *in vitro* studies to dissect its molecular mechanisms, including GTPase activity, pathogen-membrane targeting, and interactions with immune adaptors (e.g., ASC, caspases). Produced via bacterial or mammalian expression systems, it serves as a tool for structural analysis, enzymatic assays, and exploring its therapeutic potential in infectious diseases, cancer, or autoimmune disorders. Research on GBP7 also extends to understanding its dysregulation in chronic inflammation and its crosstalk with other immune pathways, highlighting its dual role as a pathogen restriction factor and a modulator of inflammatory responses.

Overall, GBP7 recombinant protein is pivotal in advancing insights into IFN-mediated immunity and developing strategies to manipulate GBP pathways for therapeutic benefit.