Recombinant Human SLC27A5 protein

中文名： 脂肪酸转运蛋白5(SLC27A5)重组蛋白
别名： SLC27A5；ACSB；ACSVL6；FACVL3；Long-chain fatty acid transport protein 5

货号: PA2000-176DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	SLC27A5
Uniprot No	Q9Y2P5
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-690aa
氨基酸序列	MGVRQQLALLLLLLLLLWGLGQPVWPVAVALTLRWLLGDPTCCVLLGLAM LARPWLGPWVPHGLSLAAAALALTLLPARLPPGLRWLPADVIFLAKILHL GLKIRGCLSRQPPDTFVDAFERRARAQPGRALLVWTGPGAGSVTFGELDA RACQAAWALKAELGDPASLCAGEPTALLVLASQAVPALCMWLGLAKLGCP TAWINPHGRGMPLAHSVLSSGARVLVVDPDLRESLEEILPKLQAENIRCF YLSHTSPTPGVGALGAALDAAPSHPVPADLRAGITWRSPALFIYTSGTTG LPKPAILTHERVLQMSKMLSLSGATADDVVYTVLPLYHVMGLVVGILGCL DLGATCVLAPKFSTSCFWDDCRQHGVTVILYVGELLRYLCNIPQQPEDRT HTVRLAMGNGLRADVWETFQQRFGPIRIWEVYGSTEGNMGLVNYVGRCGA LGKMSCLLRMLSPFELVQFDMEAAEPVRDNQGFCIPVGLGEPGLLLTKVV SQQPFVGYRGPRELSERKLVRNVRQSGDVYYNTGDVLAMDREGFLYFRDR LGDTFRWKGENVSTHEVEGVLSQVDFLQQVNVYGVCVPGCEGKVGMAAVQ LAPGQTFDGEKLYQHVRAWLPAYATPHFIRIQDAMEVTSTFKLMKTRLVR EGFNVGIVVDPLFVLDNRAQSFRPLTAEMYQAVCEGTWRL
预测分子量	75,3 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SLC27A5重组蛋白的3篇参考文献及其摘要概括：

1. **《Recombinant SLC27A5 (FATP5) characterization and its role in hepatic fatty acid transport》**

- **作者**：Smith J, Doe R, et al.

- **摘要**：研究通过重组表达SLC27A5蛋白，验证其在肝细胞中的长链脂肪酸摄取功能，并揭示其依赖ATP的转运机制。

2. **《Expression and purification of functional human SLC27A5 in a bacterial system》**

- **作者**：Chen L, Wang X, et al.

- **摘要**：开发了一种在大肠杆菌中高效表达可溶性SLC27A5重组蛋白的方法，并证实其与胆汁酸合成相关的酶活性。

3. **《Structural insights into SLC27A5 through recombinant protein crystallography》**

- **作者**：Zhang Y, Tanaka K, et al.

- **摘要**：利用重组SLC27A5蛋白进行X射线晶体学分析，解析其三维结构，阐明底物结合域的关键氨基酸残基。

(注：上述文献为示例，实际引用需根据具体研究补充真实来源。)

背景信息

SLC27A5. also known as fatty acid transport protein 5 (FATP5) or very long-chain acyl-CoA synthetase 6 (ACSVL6), is a member of the solute carrier family 27 (SLC27) involved in fatty acid metabolism. This transmembrane protein is predominantly expressed in the liver, where it localizes to the basolateral membrane of hepatocytes. It functions as a bifunctional transporter-enzyme, facilitating the uptake of long-chain fatty acids (LCFAs) and activating them via acyl-CoA synthetase activity, a critical step for their metabolic utilization in lipid synthesis, oxidation, or storage. SLC27A5 plays a key role in regulating systemic lipid homeostasis and bile acid conjugation.

Recombinant SLC27A5 protein is engineered for in vitro studies to investigate its structural, functional, and regulatory mechanisms. Produced using heterologous expression systems such as mammalian cells, insect cells, or Escherichia coli, the recombinant protein retains enzymatic activity and substrate specificity. Its production enables detailed biochemical assays, including fatty acid transport kinetics, substrate specificity profiling, and inhibitor screening. Researchers utilize it to study interactions with cofactors, membrane partners, or regulatory molecules, as well as to model metabolic disorders like non-alcoholic fatty liver disease (NAFLD) or hyperlipidemia. Additionally, recombinant SLC27A5 serves as a tool for drug discovery, aiding in the identification of therapeutic agents targeting lipid metabolism disorders. Quality assessments typically involve SDS-PAGE, Western blotting, and functional validation via acyl-CoA synthetase activity assays.