纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CK18 |
Uniprot No | P05783 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-430aa |
氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGEFSFTTRSTFSTNYRSLGSVQAPS YGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAG MGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQV RDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELA MRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKG LQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQ QIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENS LREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKL EAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETN DTKVLRH |
预测分子量 | 51 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK18重组蛋白的3篇参考文献示例(内容为虚构,仅供参考):
1. **文献名称**:*Recombinant Human Keratin 18: Expression, Purification, and Application in Cytoskeletal Studies*
**作者**:Eriksson JE, et al.
**摘要**:本研究描述了在大肠杆菌中高效表达和纯化重组人CK18蛋白的方法,并利用体外组装实验验证其形成中间丝的能力,为细胞骨架动态研究提供工具。
2. **文献名称**:*CK18 as a Biomarker in Liver Disease: Development of a Recombinant Protein-Based ELISA Assay*
**作者**:Omary MB, et al.
**摘要**:通过重组CK18蛋白制备抗体,建立定量检测血清中CK18片段的新型ELISA方法,验证其在肝纤维化和肝癌早期诊断中的潜在应用价值。
3. **文献名称**:*Structural Insights into Recombinant CK18 Phosphorylation and Apoptosis Regulation*
**作者**:Leers MP, et al.
**摘要**:利用重组CK18蛋白分析其磷酸化位点对细胞凋亡的影响,揭示Caspase介导的CK18切割机制及其在程序性死亡中的信号传递作用。
4. **文献名称**:*Recombinant CK18 in Drug Screening: Targeting Intermediate Filaments for Cancer Therapy*
**作者**:Herrmann H, et al.
**摘要**:基于重组CK18蛋白建立高通量药物筛选平台,发现小分子化合物可特异性破坏CK18组装,为靶向上皮源性肿瘤的疗法提供新策略。
(注:以上文献及作者为模拟生成,实际引用请核实真实数据库。)
Cytokeratin 18 (CK18), a type I intermediate filament protein, is a key structural component of epithelial cells. It is predominantly expressed in single-layered or "simple" epithelial tissues, such as those in the liver, gastrointestinal tract, and glandular organs. As part of the keratin family, CK18 pairs with its type II partner, cytokeratin 8 (CK8), to form heteropolymers that maintain cellular integrity, resist mechanical stress, and regulate signaling pathways. Its expression is often dysregulated in epithelial-derived cancers (e.g., hepatocellular carcinoma, breast cancer), making it a biomarker for disease progression and epithelial-mesenchymal transition.
Recombinant CK18 protein is produced using genetic engineering techniques, typically by inserting the human KRT18 gene into bacterial (e.g., E. coli) or mammalian expression systems. This allows large-scale production of purified CK18 for research and diagnostic applications. Post-translational modifications, such as phosphorylation during apoptosis, can be preserved in mammalian-derived recombinant CK18. enhancing its functional relevance.
Clinically, CK18 fragments released during cell death are detectable in serum. Antibodies targeting caspase-cleaved CK18 (M30 epitope) or total CK18 (M65 epitope) are used to assess apoptosis and necrosis in liver diseases, chemotherapy response, and toxicological studies. In research, recombinant CK18 aids in studying epithelial cell behavior, tumor metastasis mechanisms, and drug screening. Its role in forming cytoplasmic networks also makes it a tool for exploring cytoskeletal dynamics and cellular stress responses. Despite its utility, interpretation requires caution due to tissue-specific expression patterns and variation in post-translational states across pathologies.
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