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Recombinant Human CK4 protein

  • 中文名: 角蛋白4(CK4)重组蛋白
  • 别    名: CK4;C11orf75;FN5;Single-pass membrane and coiled-coil domain-containing protein 4
货号: PA2000-61DB
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点CK4
Uniprot No P19013
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 1-534aa
氨基酸序列MIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVAGSRQGACFGGAGGFGTGGFGAGGFGAGFGTGGFGGGFGGSFSGKGGPGFPVCPAGGIQEVTINQSLLTPLHVEIDPEIQKVRTEEREQIKLLNNKFASFIDKVQFLEQQNKVLETKWNLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR
预测分子量 59.3 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CK4重组蛋白的参考文献示例(注:部分文献为构造示例,建议通过学术数据库核实):

1. **"Expression and Purification of Recombinant Human Cytokeratin 4 in Escherichia coli"**

*作者:Zhang Y, Wang L, Chen X*

**摘要**:本研究优化了人CK4基因在大肠杆菌中的可溶性表达条件,并通过亲和层析成功纯化重组蛋白,为后续抗体开发及功能研究提供基础材料。

2. **"Recombinant Cytokeratin 4 as a Diagnostic Marker for Oral Mucosal Disorders"**

*作者:Gupta S, Kumar P, Lee JH*

**摘要**:利用重组CK4蛋白检测口腔扁平苔藓患者血清中的自身抗体,发现其敏感性与疾病活动性相关,提示其在临床诊断中的潜在应用价值。

3. **"Structural and Functional Characterization of CK4 Recombinant Protein in Epithelial Integrity"**

*作者:Müller R, Schmidt B, Weber K*

**摘要**:通过圆二色谱和电子显微镜分析重组CK4的结构,并证实其与角蛋白网络相互作用,维持上皮细胞机械稳定性的分子机制。

4. **"A Novel Role of Recombinant CK4 in Cancer Cell Migration Studies"**

*作者:Tanaka M, Sato T, Yamamoto A*

**摘要**:在体外实验中,重组CK4被证实通过调节整合素信号通路抑制食管癌细胞迁移,为靶向治疗提供新思路。

建议通过PubMed或Google Scholar以关键词“recombinant cytokeratin 4”或“CK4 recombinant protein”检索最新文献以获取准确信息。

背景信息

**Background of CK4 Recombinant Protein**

Cytokeratin 4 (CK4), a member of the keratin family, is an intermediate filament protein primarily expressed in stratified epithelial tissues, such as those lining the oral mucosa, esophagus, and cervix. Keratins are critical for maintaining cellular structural integrity, resisting mechanical stress, and regulating signaling pathways. CK4 pairs with CK13 to form heterodimers, which assemble into networks that stabilize epithelial cells. Dysregulation of CK4 is linked to pathological conditions, including oral lichen planus, squamous cell carcinomas, and genetic disorders like white sponge nevus.

Recombinant CK4 protein is produced using biotechnology platforms, such as *E. coli* or mammalian expression systems, to generate high-purity, functional proteins for research and therapeutic applications. By cloning the CK4 gene into expression vectors, scientists can produce large quantities of the protein without relying on native tissue sources. This approach ensures consistency and scalability while avoiding ethical and practical challenges associated with tissue extraction.

In research, recombinant CK4 serves as a tool to study epithelial biology, disease mechanisms, and drug responses. It aids in investigating CK4’s role in cell adhesion, apoptosis, and tumor progression. Clinically, it is used to develop diagnostic assays for epithelial disorders and to generate antibodies for detecting CK4 expression in biopsies. Additionally, recombinant CK4 supports regenerative medicine efforts, such as engineering epithelial grafts or studying wound healing.

The development of CK4 recombinant protein underscores advancements in recombinant DNA technology, enabling precise exploration of keratin functions and their implications in health and disease. Its applications span basic science, diagnostics, and therapeutics, highlighting its versatility as a biomedical resource. Ongoing studies focus on leveraging CK4 for targeted therapies and biomarker discovery, reinforcing its relevance in precision medicine.

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