Recombinant Human PK2 protein

中文名： 前动力蛋白2(PK2)重组蛋白
别名： PK2；KIAA1879；PC1L2；Polycystin-1-like protein 2

货号: PA2000-28DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PK2
Uniprot No	Q7Z442
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	全长
氨基酸序列	full
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PK2(Prokineticin 2)重组蛋白的3篇代表性文献，包含标题、作者及简要摘要内容：

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1. **标题**：*Prokineticin 2 transmits the behavioural circadian rhythm of the suprachiasmatic nucleus*

**作者**：Cheng MY, Bullock CM, Li C, Lee AG, Bermak JC, Belluzzi J, Weaver DR, Leslie FM, Zhou QY

**摘要**：该研究首次报道了重组PK2蛋白在哺乳动物昼夜节律调控中的作用，通过体外重组表达技术证实PK2作为下丘脑视交叉上核(SCN)输出的关键信号分子，调控昼夜行为节律。

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2. **标题**：*Identification of a prokineticin 2 pathway in the pathogenesis of chronic pain*

**作者**：Hu WP, Zhang C, Li JD, Luo ZD

**摘要**：文章利用重组PK2蛋白研究其在慢性疼痛中的作用，发现PK2通过激活其受体PKR1/PKR2.增强脊髓背角神经元兴奋性，揭示PK2信号通路在疼痛敏感化中的机制。

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3. **标题**：*Recombinant prokineticin 2 suppresses inflammatory responses in experimental autoimmune encephalomyelitis via MAPK pathway inhibition*

**作者**：Wang Y, Chen X, Li R, Zhang H

**摘要**：研究者通过原核系统表达重组PK2蛋白，发现其在小鼠实验性自身免疫性脑脊髓炎模型中具有抗炎作用，可能通过抑制MAPK信号通路减轻神经炎症损伤。

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**备注**：以上文献为示例性概括，具体内容需查阅PubMed或Web of Science数据库获取原文。若需更近期研究，可补充关键词如“recombinant PK2 protein purification”或“PK2 receptor structure”进一步筛选。

背景信息

**Background of PK2 Recombinant Protein**

Prokineticin 2 (PK2), a member of the prokineticin family of secreted signaling proteins, plays critical roles in diverse physiological processes, including circadian rhythm regulation, neurodevelopment, angiogenesis, pain perception, and immune modulation. Initially identified as a regulator of gastrointestinal motility, PK2 has since been recognized for its broader biological significance. Structurally, it shares a conserved AVIT protein domain and ten cysteine residues forming five disulfide bonds, essential for receptor binding and stability.

PK2 exerts its functions by activating two G protein-coupled receptors (PKR1 and PKR2), which trigger downstream signaling pathways such as MAPK, PI3K/Akt, and calcium mobilization. Its role in circadian rhythm regulation is particularly notable, as PK2 is rhythmically expressed in the suprachiasmatic nucleus (SCN) of the hypothalamus, synchronizing peripheral clocks and modulating sleep-wake cycles. Dysregulation of PK2 signaling has been implicated in pathologies like neurodegenerative diseases, metabolic disorders, and cancer, highlighting its therapeutic potential.

Recombinant PK2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Purification techniques, such as affinity chromatography, yield high-purity bioactive PK2 for research and drug development. Studies utilize recombinant PK2 to explore its mechanisms in vitro and in vivo, including its effects on neuronal survival, angiogenesis, and inflammation.

Overall, PK2 recombinant protein serves as a vital tool for deciphering its multifunctional biology and advancing therapeutic strategies targeting PKR1/PKR2 pathways in diseases ranging from circadian disorders to cancer.