| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Des |
| Uniprot No | P17661 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-470aa |
| 氨基酸序列 | SQAYSSSQRVSSYRRTFGGAPGFPLGSPLSSPVFPRAGFGSKGSSSSVTSRVYQVSRTSGGAGGLGSLRASRLGTTRTPSSYGAGELLDFSLADAVNQEFLTTRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVNRLKGREPTRVAELYEEELRELRRQVEVLTNQRARVDVERDNLLDDLQRLKAKLQEEIQLKEEAENNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEASGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL |
| 预测分子量 | 80.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Des重组蛋白的3篇假设参考文献，供参考：
1. **"High-Yield Expression and Purification of Recombinant Human Desmin in Escherichia coli"**
- 作者：Müller, R., et al.
- 摘要：研究提出了一种优化的大肠杆菌表达系统，用于高效生产重组人Desmin蛋白，并通过色谱技术实现高纯度制备，为体外研究Desmin的生化特性提供可靠方法。
2. **"Recombinant Desmin Filament Assembly: Implications for Myopathies"**
- 作者：Bär, H., et al.
- 摘要：探讨重组Desmin蛋白在体外自组装成中间丝的过程，揭示特定突变如何破坏其聚合能力，从而解释遗传性Desmin相关肌病的分子机制。
3. **"Functional Characterization of Mutant Recombinant Desmin in Cardiomyocytes"**
- 作者：Goldfarb, L.G., et al.
- 摘要：通过将突变型重组Desmin导入心肌细胞，研究其异常聚集对细胞机械稳定性的影响，为扩张型心肌病的治疗策略提供新见解。
4. **"Structural Insights into Recombinant Desmin Binding Partners Using Cryo-EM"**
- 作者：Strelkov, S.V., et al.
- 摘要：利用冷冻电镜解析重组Desmin与αB-晶状体蛋白的复合物结构，阐明分子伴侣在调控Desmin纤维组装中的关键作用。
(注：上述文献为示例，实际引用时请核实真实文献信息。)
**Background of Recombinant Des Protein**
Recombinant Des protein refers to a genetically engineered form of desmin, a key intermediate filament protein predominantly expressed in muscle cells. Desmin plays a critical role in maintaining structural integrity, mechanical stability, and functional organization of muscle fibers by connecting the cytoskeleton to contractile machinery and organelles. Mutations in the *DES* gene encoding desmin are linked to desminopathies, a group of neuromuscular disorders characterized by muscle weakness, cardiomyopathy, and respiratory dysfunction.
The development of recombinant Des protein emerged from the need to study its biochemical properties, interactions, and disease-related mechanisms in vitro. Traditional methods of isolating desmin from tissues were limited by low yield and purity. Recombinant technology allows large-scale production in bacterial (e.g., *E. coli*) or eukaryotic expression systems, enabling precise control over post-translational modifications and mutant variants.
Research applications include investigating desmin’s role in cytoskeletal dynamics, its aggregation in myopathies, and interactions with binding partners like synemin or αB-crystallin. Recombinant Des protein is also used to generate antibodies for diagnostic tools and to screen potential therapeutics targeting desmin aggregation. Challenges remain in mimicking native folding and phosphorylation states, necessitating optimized expression and purification strategies.
Overall, recombinant Des protein serves as a vital tool for advancing our understanding of muscle biology and developing treatments for desmin-related diseases. Its utility underscores the intersection of genetic engineering and molecular pathology in addressing neuromuscular disorders.
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