Recombinant Human TCHH protein

中文名： 毛透明蛋白(TCHH)重组蛋白
别名： TCHH；THH；THL；TRHY；Trichohyalin

货号: PA1000-9603

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	TCHH
Uniprot No	Q07283
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1854-1943aa
氨基酸序列	QEKSRREEQELWQEEEQKRRQERERKLREEHIRRQQKEEQRHRQVGEIKSQEGKGHGRLLEPGTHQFASVPVRSSPLYEYIQEQRSQYRP
预测分子量	18.6 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与TCHH(Trichohyalin)重组蛋白相关的模拟文献摘要(注：以下内容为基于领域知识的模拟，非真实文献)：

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1. **文献名称**: *"Recombinant Trichohyalin Production and Its Role in Hair Follicle Keratinization"*

**作者**: Smith A, et al.

**摘要**: 研究利用大肠杆菌系统表达重组TCHH蛋白，通过体外实验证实TCHH通过交联角蛋白中间纤维参与毛囊角质化过程，并发现其钙离子依赖性酶活性对毛发结构稳定性至关重要。

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2. **文献名称**: *"Structural Analysis of Trichohyalin in Epidermal Differentiation Using Recombinant Protein Models"*

**作者**: Lee J, et al.

**摘要**: 通过杆状病毒表达系统制备重组TCHH蛋白，结合冷冻电镜解析其α-螺旋结构域，揭示TCHH通过形成二聚体与角蛋白KRT75相互作用，调控表皮分化中细胞骨架重排的分子机制。

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3. **文献名称**: *"TCHH Gene Mutations and Recombinant Protein Rescue in Hereditary Hair Disorders"*

**作者**: Johnson R, et al.

**摘要**: 在遗传性毛发疾病患者中发现TCHH功能缺失突变，利用哺乳动物细胞表达的重组TCHH蛋白成功恢复突变细胞模型的角蛋白交联功能，为基因治疗提供潜在策略。

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4. **文献名称**: *"Functional Characterization of Recombinant Trichohyalin in Skin Barrier Development"*

**作者**: Brown K, et al.

**摘要**: 通过体外重建实验证明重组TCHH蛋白可增强表皮屏障的机械强度，并发现其通过激活转谷氨酰胺酶途径促进角质包膜形成，为皮肤屏障修复研究提供新靶点。

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**备注**：实际研究中建议通过PubMed或Google Scholar检索关键词"Trichohyalin recombinant protein"或结合疾病背景(如毛发疾病、皮肤屏障)筛选文献。

背景信息

**Background of TCHH Recombinant Protein**

Trichohyalin (TCHH) is a structural scaffold protein primarily expressed in the inner root sheath (IRS) of hair follicles and the granular layer of the epidermis. It plays a critical role in maintaining the mechanical integrity of hair and skin by interacting with keratin intermediate filaments and other structural proteins. TCHH undergoes post-translational modifications, including phosphorylation and citrullination, which facilitate its cross-linking to the cell cytoskeleton during terminal differentiation of epithelial cells.

Recombinant TCHH protein is engineered to study its biological functions and applications in vitro or in vivo. It is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and activity. Research on TCHH has focused on its role in hair follicle development, epidermal barrier formation, and wound healing. Mutations or dysregulation of TCHH are linked to hair disorders (e.g., uncombable hair syndrome) and skin pathologies, making it a potential therapeutic target.

In biomedical applications, recombinant TCHH is utilized to investigate cell adhesion, differentiation, and tissue engineering strategies for skin or hair regeneration. It also serves as an antigen for antibody development in diagnostic assays. Despite progress, challenges remain in understanding its precise molecular interactions and post-translational regulation. Further studies on TCHH could advance therapies for ectodermal dysplasias and cosmetic innovations in hair care.