首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子

Recombinant Human MPIF1 protein

  • 中文名: 髓样前体细胞抑制因子1(MPIF1)重组蛋白
  • 别    名: MPIF1;MIP3;MPIF1;SCYA23;C-C motif chemokine 23
货号: PA1000-9190
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MPIF1
Uniprot No P55773
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间22-120aa
氨基酸序列RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
预测分子量 11.4 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MPIF1重组蛋白的3篇参考文献示例(注:内容为模拟概括,实际文献需通过学术数据库查询):

---

1. **文献名称**: *"Cloning and functional characterization of recombinant human MPIF-1. a novel CC chemokine inhibitor of myeloid progenitor proliferation"*

**作者**: Broxmeyer HE, et al.

**摘要概括**: 本研究报道了人源MPIF1重组蛋白的克隆与表达,发现其能特异性抑制髓系祖细胞的体外增殖,揭示了其在造血调控中的潜在作用,并通过受体结合实验证实其与CCR1受体的相互作用。

2. **文献名称**: *"MPIF-1/Chemokine CC motif ligand 23 (CCL23) acts as a selective agonist for CCR1 in inflammatory models"*

**作者**: Menten P, et al.

**摘要概括**: 利用重组MPIF1蛋白探究其趋化活性,发现其通过结合CCR1受体介导单核细胞迁移,且在炎症模型中抑制中性粒细胞浸润,提示其在调控免疫细胞募集中的功能。

3. **文献名称**: *"Structural and functional analysis of recombinant MPIF-1 highlights its role in tumor-associated macrophage modulation"*

**作者**: Liu Y, et al.

**摘要概括**: 通过重组MPIF1蛋白的结构解析,阐明了其与受体结合的关键结构域,并证明其在肿瘤微环境中可抑制巨噬细胞的促肿瘤活性,为癌症治疗提供了新靶点。

---

如需具体文献,建议通过PubMed或Web of Science以关键词“MPIF1 recombinant”“CCL23”“CC chemokine”检索近年研究。

背景信息

**Background of MPIF1 Recombinant Protein**

MPIF1 (Myeloid Progenitor Inhibitory Factor 1), also known as CCL23. is a chemokine belonging to the CC subfamily. It is primarily secreted by myeloid cells, such as monocytes and macrophages, and plays a regulatory role in immune responses by influencing leukocyte migration, activation, and differentiation. Initially identified for its ability to inhibit the proliferation of hematopoietic progenitor cells, MPIF1 is implicated in modulating inflammation, hematopoiesis, and tumor microenvironment interactions.

The recombinant MPIF1 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This engineered form retains the functional properties of native MPIF1. including binding to its cognate receptor CCR1. which triggers downstream signaling pathways like MAPK and PI3K/Akt. Such interactions enable MPIF1 to regulate immune cell recruitment and inflammatory processes.

Research highlights MPIF1's dual role: it may suppress excessive inflammation in autoimmune diseases while promoting tumor progression in certain cancers by enhancing angiogenesis or immune evasion. Its recombinant variant is widely used in *in vitro* and *in vivo* studies to explore these mechanisms, offering potential therapeutic avenues for conditions like rheumatoid arthritis, atherosclerosis, or cancer.

Overall, MPIF1 recombinant protein serves as a critical tool for dissecting chemokine biology and developing targeted immunotherapies.

客户数据及评论

折叠内容

大包装询价

×