Recombinant Human CYP11B1 protein

中文名： 细胞色素P450家族成员11B1(CYP11B1)重组蛋白
别名： CYP11B1；S11BH；Cytochrome P450 11B1, mitochondrial

货号: PA1000-9023

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	CYP11B1
Uniprot No	P15538
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	25-503aa
氨基酸序列	GTRAARVPRTVLPFEAMPRRPGNRWLRLLQIWREQGYEDLHLEVHQTFQELGPIFRYDLGGAGMVCVMLPEDVEKLQQVDSLHPHRMSLEPWVAYRQHRGHKCGVFLLNGPEWRFNRLRLNPEVLSPNAVQRFLPMVDAVARDFSQALKKKVLQNARGSLTLDVQPSIFHYTIEASNLALFGERLGLVGHSPSSASLNFLHALEVMFKSTVQLMFMPRSLSRWTSPKVWKEHFEAWDCIFQYGDNCIQKIYQELAFSRPQQYTSIVAELLLNAELSPDAIKANSMELTAGSVDTTVFPLLMTLFELARNPNVQQALRQESLAAAASISEHPQKATTELPLLRAALKETLRLYPVGLFLERVASSDLVLQNYHIPAGTLVRVFLYSLGRNPALFPRPERYNPQRWLDIRGSGRNFYHVPFGFGMRQCLGRRLAEAEMLLLLHHVLKHLQVETLTQEDIKMVYSFILRPSMFPLLTFRAIN
预测分子量	61.9 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CYP11B1重组蛋白的3篇参考文献的简要信息(基于公开研究整理，部分内容可能非最新文献)：

1. **文献名称**：*"Heterologous expression and characterization of human CYP11B1 in Saccharomyces cerevisiae"*

**作者**：Kawamoto T. et al.

**摘要**：研究利用酵母表达系统成功表达人源CYP11B1重组蛋白，并验证其催化11β-羟化酶活性，证实其在皮质醇合成中的功能，为类固醇代谢机制研究提供模型。

2. **文献名称**：*"Purification and functional analysis of recombinant CYP11B1: role in glucocorticoid biosynthesis"*

**作者**：Denner K. et al.

**摘要**：通过大肠杆菌系统表达并纯化CYP11B1重组蛋白，结合体外酶活实验，证明其依赖NADPH的羟化酶活性，并探讨突变体对酶功能的潜在影响。

3. **文献名称**：*"Structural insights into CYP11B1 for substrate specificity using homology modeling"*

**作者**：Peterson J.A. et al.

**摘要**：基于重组CYP11B1蛋白的生化数据构建同源模型，分析其底物结合口袋结构特征，揭示其与CYP11B2的差异，解释两者在类固醇合成中的不同催化偏好性。

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**说明**：以上文献为示例性内容，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“CYP11B1 recombinant expression/purification/structural analysis”查找最新研究。

背景信息

CYP11B1. also known as steroid 11β-hydroxylase, is a mitochondrial cytochrome P450 enzyme critical in cortisol biosynthesis. Primarily expressed in the adrenal cortex, it catalyzes the conversion of 11-deoxycortisol to cortisol—a key glucocorticoid regulating metabolism, immune response, and stress adaptation. This enzyme also participates in the synthesis of corticosterone from 11-deoxycorticosterone. Its activity is tightly controlled by adrenocorticotropic hormone (ACTH) through cAMP signaling pathways.

Recombinant CYP11B1 protein is engineered using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to overcome challenges in isolating native forms from adrenal tissues. The recombinant version retains catalytic activity while enabling scalable production for biochemical studies. Researchers often co-express it with adrenodoxin reductase and adrenodoxin to reconstitute its electron transport system, as CYP11B1 requires these partner proteins for hydroxylation reactions.

This recombinant tool has become essential for investigating enzyme kinetics, substrate specificity, and inhibition mechanisms. It plays a pivotal role in studying congenital adrenal hyperplasia (CAH) caused by CYP11B1 mutations, which leads to cortisol deficiency and androgen excess. Additionally, pharmaceutical research leverages recombinant CYP11B1 to screen 11β-hydroxylase inhibitors for treating Cushing's syndrome and hypertension. Recent structural studies using recombinant protein have revealed insights into its substrate-binding pocket and interactions with isoform-specific inhibitors, distinguishing it from the highly homologous aldosterone synthase (CYP11B2). These advances highlight its dual significance in basic endocrinology and therapeutic development.