Recombinant Human TNPO1 protein

中文名： 转运蛋白1(TNPO1)重组蛋白
别名： TNPO1；KPNB2；MIP1；TRN；Transportin-1

货号: PA1000-8629

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	TNPO1
Uniprot No	Q92973-2
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	25-141aa
氨基酸序列	PDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKN NVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQ NWPDLLPKLCSLLDSED*
预测分子量	39 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TNPO1重组蛋白的参考文献示例(内容为模拟生成，建议通过学术数据库验证准确性)：

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1. **文献名称**: *TNPO1 facilitates HIV-1 nuclear import by interacting with viral integrase*

**作者**: Christ F. et al.

**摘要**: 本研究利用重组TNPO1蛋白，揭示了其通过与HIV-1整合酶直接结合，促进病毒基因组核转运的机制。体外实验表明，TNPO1的C端结构域对病毒核导入至关重要，为抗病毒靶点开发提供了依据。

2. **文献名称**: *Structural insights into Transportin-1 mediated nuclear import of splicing factors*

**作者**: Imasaki T. et al.

**摘要**: 通过重组TNPO1蛋白的晶体结构解析，研究发现其通过特定的HEAT重复结构域识别携带脯氨酸-酪氨酸核定位信号的蛋白(如SR蛋白)，阐明了TNPO1在剪接因子核转运中的分子机制。

3. **文献名称**: *TNPO1 dysfunction in C9orf72-ALS/FTD disrupts nucleocytoplasmic transport*

**作者**: Zhang K. et al.

**摘要**: 该研究利用重组TNPO1蛋白进行体外结合实验，证明C9orf72基因突变导致二肽重复蛋白异常聚集，干扰TNPO1介导的核质运输功能，揭示了肌萎缩侧索硬化症的潜在病理机制。

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**备注**：以上为基于TNPO1相关研究的典型方向模拟的文献，实际文献请通过PubMed或Google Scholar以关键词“TNPO1 recombinant protein”检索，并关注近年结构生物学、病毒学及神经退行性疾病领域的研究。

背景信息

**Background of TNPO1 Recombinant Protein**

TNPO1 (Transportin-1), also known as Importin-β2 or karyopherin-β2. is a member of the importin-β family of nuclear transport receptors. It plays a critical role in the nucleocytoplasmic transport of proteins, facilitating their entry into the nucleus by recognizing cargoes bearing specific proline-tyrosine (PY)-nuclear localization signals. TNPO1 is essential for cellular processes such as gene expression regulation, RNA processing, and cell cycle progression.

Structurally, TNPO1 contains characteristic HEAT repeats that mediate interactions with cargo proteins and components of the nuclear pore complex. Beyond its canonical transport role, TNPO1 has gained attention for its involvement in HIV-1 replication, where it assists in the nuclear import of viral pre-integration complexes. Additionally, TNPO1 interacts with proteins linked to neurodegenerative diseases, including FUS and TDP-43. whose mislocalization is associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia.

Recombinant TNPO1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality. It serves as a vital tool for studying nuclear transport mechanisms, virus-host interactions, and disease pathology. Researchers employ TNPO1 recombinant protein in *in vitro* binding assays, structural studies, and screening for inhibitors that could modulate nuclear transport pathways. Its applications extend to exploring therapeutic strategies for viral infections and neurodegenerative disorders by targeting TNPO1-dependent transport processes.

Overall, TNPO1 recombinant protein bridges fundamental research and translational studies, offering insights into cellular dynamics and potential avenues for drug development.