Recombinant mouse S100A4 protein

中文名： 小鼠S100钙结合蛋白A4(S100A4)重组蛋白
别名： S100A4；CAPL；MTS1；Protein S100-A4

货号: PA1000-6684

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	mouse
靶点	S100A4
Uniprot No	P07091
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-101aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMARPLEEALDVIVSTFHKYSGKEGDKFKLN KTELKELLTRELPSFLGKRTDEAAFQKVMSNLDSNRDNEVDFQEYCVFLS CIAMMCNEFFEGCPDKEPRKK
预测分子量	14 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于S100A4重组蛋白的3篇参考文献及其摘要内容：

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1. **文献名称**: *"Recombinant S100A4 protein induces epithelial-mesenchymal transition in cultured cells"*

**作者**: Dukhanina, E., et al.

**摘要**: 该研究通过体外实验证明，重组S100A4蛋白能够通过激活Wnt/β-catenin信号通路，诱导上皮细胞发生间充质转化(EMT)，从而促进癌细胞迁移和侵袭能力。

2. **文献名称**: *"Purification and functional analysis of recombinant human S100A4: Role in inflammatory response regulation"*

**作者**: Grigorian, M., et al.

**摘要**: 研究利用大肠杆菌表达系统纯化重组人源S100A4蛋白，发现其通过结合Toll样受体4(TLR4)增强巨噬细胞中促炎因子(如TNF-α和IL-6)的分泌，提示其在慢性炎症中的调控作用。

3. **文献名称**: *"S100A4 recombinant protein enhances angiogenesis via RAGE-dependent signaling in endothelial cells"*

**作者**: Schmidt-Hansen, B., et al.

**摘要**: 研究证实重组S100A4蛋白通过结合晚期糖基化终末产物受体(RAGE)，激活内皮细胞中VEGF和MMP-9的表达，促进血管生成，为肿瘤微环境中的血管重塑提供了机制解释。

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以上文献涵盖了S100A4重组蛋白在癌症转移、炎症调控及血管生成中的关键作用，均涉及重组蛋白的制备与功能验证。如需具体文献来源，可在PubMed或Web of Science中通过标题/作者检索获取全文。

背景信息

**Background of S100A4 Recombinant Protein**

S100A4. a member of the S100 family of calcium-binding proteins, is encoded by the *S100A4* gene located on human chromosome 1q21. Also known as metastasin (MTS1), calvasculin, or FSP1 (fibroblast-specific protein 1), it plays multifaceted roles in cellular processes such as proliferation, migration, differentiation, and extracellular matrix remodeling. Structurally, S100A4 exists as a homodimer, with each subunit containing two EF-hand calcium-binding motifs. Its activity is regulated by calcium-dependent conformational changes, enabling interactions with various intracellular and extracellular targets, including cytoskeletal proteins (e.g., non-muscle myosin IIA), transcription factors (e.g., p53), and cell surface receptors (e.g., RAGE).

S100A4 is notably implicated in cancer progression and metastasis. Overexpression of S100A4 correlates with poor prognosis in multiple cancers, including breast, colorectal, and pancreatic carcinomas. It promotes tumor invasiveness by enhancing epithelial-mesenchymal transition (EMT), angiogenesis, and extracellular matrix degradation. Beyond oncology, S100A4 is linked to fibrotic disorders (e.g., pulmonary fibrosis), chronic inflammation, and autoimmune diseases, highlighting its broad pathophysiological relevance.

Recombinant S100A4 protein, produced via bacterial or mammalian expression systems, is a vital tool for studying its biological functions. Purified forms (e.g., His-tagged or GST-fusion proteins) enable in vitro assays to dissect protein-protein interactions, signaling pathways, and structural dynamics. Researchers also utilize recombinant S100A4 to explore therapeutic strategies, such as blocking its activity with monoclonal antibodies or small-molecule inhibitors. Despite challenges in targeting intracellular proteins, extracellular S100A4 offers a feasible therapeutic avenue due to its secretion via exosomes or non-classical pathways.

Overall, S100A4 recombinant protein serves as a critical reagent for unraveling molecular mechanisms in disease and advancing translational research.