Recombinant Human VNN1 protein

中文名： 血管非炎性蛋白1(VNN1)重组蛋白
别名： VNN1；Pantetheinase

货号: PA1000-6045

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	VNN1
Uniprot No	O95497
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	22-490aa
氨基酸序列	QDTFTAAVYEHAAILPNATLTPVSREEALALMNRNLDILEGAITSAADQG AHIIVTPEDAIYGWNFNRDSLYPYLEDIPNPEVNWIPCNNRNRFGQTPVQ ERLSCLAKNNSIYVVANIGDKKPCDTSDPQCPPDGRYQYNTDVVFDSQGK LVARYHKQNLFMGENQFNVPKEPEIVTFNTTFGSFGIFTCFDILFHDPAV TLVKDFHVDTIVFPTAWMNVLPHLSAVEFHSAWAMGMRVNFLASNIHYPS KKMTGSGIYAPNSSRAFHYDMKTEEGKLLLSQLDSHPSHSAVVNWTSYAS SIEALSSGNKEFKGTVFFDEFTFVKLTGVAGNYTVCQKDLCCHLSYKMSE NIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTCGDSAETASTR FEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVL TVTLFGRLYEKDWASNASSVDHHHHHH
预测分子量	53 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于VNN1重组蛋白的3篇文献示例(注：内容为模拟生成，实际文献需通过学术数据库查询验证)：

1. **文献名称**：**"Recombinant human Vanin-1 (VNN1) exhibits pantetheinase activity and modulates oxidative stress in vitro"**

**作者**：Martin, F., et al.

**摘要**：研究报道了重组人VNN1蛋白的制备与功能验证，证实其具有泛酸酶活性，能够催化泛酰巯基乙胺分解，并在细胞模型中减轻氧化应激损伤，提示其在炎症相关疾病中的潜在调控作用。

2. **文献名称**：**"Structural characterization of recombinant murine VNN1 and its role in hepatic lipid metabolism"**

**作者**：Yamazaki, T., et al.

**摘要**：通过重组表达的小鼠VNN1蛋白，解析了其晶体结构，揭示了关键催化位点;实验进一步发现该蛋白通过调节脂质代谢相关通路，影响肝脏脂肪堆积，为代谢综合征的治疗提供了新靶点。

3. **文献名称**：**"Recombinant VNN1 enhances intestinal barrier function via PPARγ signaling in a colitis model"**

**作者**：Garcia, S., et al.

**摘要**：研究利用重组VNN1蛋白处理结肠炎模型，证明其通过激活PPARγ通路增强肠道上皮屏障完整性，减少炎症因子释放，为炎症性肠病的治疗提供了实验依据。

如需具体文献，建议通过PubMed或Web of Science检索关键词“VNN1 recombinant protein”或“recombinant Vanin-1”获取最新研究。

背景信息

**Background of VNN1 Recombinant Protein**

VNN1 (Vanin-1) is a glycosylphosphatidylinositol (GPI)-anchored membrane protein encoded by the *VNN1* gene, belonging to the vanin family of pantetheinase enzymes. It is widely expressed in tissues such as the liver, kidney, and intestine, and plays a pivotal role in oxidative stress response, inflammation, and cellular metabolism. VNN1 catalyzes the hydrolysis of pantetheine to produce cysteamine and pantothenic acid (vitamin B5), linking it to glutathione metabolism and the regulation of redox homeostasis.

The recombinant VNN1 protein is engineered using biotechnological platforms (e.g., mammalian, insect, or bacterial expression systems) to produce a purified, bioactive form for research and therapeutic applications. Its recombinant form retains enzymatic activity, enabling studies on its interaction with substrates, inhibitors, or signaling pathways.

VNN1 has garnered attention for its dual role in health and disease. It modulates immune responses by influencing leukocyte migration and adhesion, and its expression is upregulated during inflammation, tissue injury, or metabolic disorders like diabetes and obesity. Conversely, VNN1 deficiency in models correlates with reduced oxidative damage and improved metabolic profiles, highlighting its therapeutic potential.

In drug discovery, recombinant VNN1 serves as a tool to screen inhibitors or activators targeting pantetheinase activity. It also holds promise as a biomarker for inflammatory or metabolic conditions. Ongoing research explores its involvement in gut barrier function, cancer progression, and neurodegenerative diseases, underscoring its multifaceted biological significance.