关键信息
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基因名
EphA4
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简介
The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293, His) is the recombinant human-derived EphA4 protein, expressed by HEK293, with C-His labeled tag.
- 应用
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别名
rHuEphrin type-A receptor 4/EphA4; Ephrin type-A receptor 4; HEK8; SEK; TYRO1; EPHA4; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1; EK8; hEK8; EPH-like kinase 8
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54764-1
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表达区间
V20-T547
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蛋白长度
Partial
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分子量
65-80 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EphA4, a member of the ephrin receptor family, plays a crucial role in various biological processes, including cell adhesion, migration, and neural development. Its interaction with ephrin ligands is significant in mediating bidirectional signaling pathways that influence cellular behaviors in different tissues. Dysregulation of EphA4 has been implicated in several pathologies, including cancer, neurological disorders, and cardiovascular diseases, making it an important target for therapeutic interventions. Research on recombinant EphA4 protein has gained momentum due to the potential of modulating its signaling pathways for clinical applications. By generating and studying recombinant EphA4, scientists aim to better understand its functional mechanisms and explore its role in disease progression and treatment. Techniques such as protein engineering and expression in various systems facilitate the production of functional EphA4 proteins, which can be used for binding assays, structural studies, and potential drug development. Moreover, understanding EphA4's molecular structure and its interactions with ligands could lead to the design of selective inhibitors or agonists, providing new strategies for combating diseases associated with EphA4 dysregulation. Overall, the study of recombinant EphA4 protein is pivotal in unveiling its biological significance and advancing therapeutic approaches targeting EphA4-related pathways.
