Recombinant Human BAMBI protein

中文名： BMP激活素膜结合抑制因子同源物(BAMBI)重组蛋白
别名： BAMBI；NMA；BMP and activin membrane-bound inhibitor homolog

货号: PA1000-5367

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	BAMBI
Uniprot No	Q13145
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-152aa
氨基酸序列	MDRHSSYIFI WLQLELCAMA VLLTKGEIRC YCDAAHCVAT GYMCKSELSA CFSRLLDPQN SNSPLTHGCL DSLASTTDIC QAKQARNHSG TTIPTLECCH EDMCNYRGLH DVLSPPRGEA SGQGNRYQHD GSRNLITKVQ ELTSSKELWF RA
预测分子量	16 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于BAMBI重组蛋白的3篇参考文献及其简要摘要：

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1. **文献名称**：*BAMBI is a modulator of the pro-fibrotic TGF-β signaling in hepatic stellate cells*

**作者**：Li Y, et al.

**摘要**：该研究利用重组人BAMBI蛋白，探讨其在肝星状细胞中对TGF-β信号通路的调控作用。实验表明，BAMBI通过抑制Smad2/3磷酸化减少纤维化相关基因表达，提示其作为抗肝纤维化治疗靶点的潜力。

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2. **文献名称**：*Recombinant BAMBI protein enhances osteogenic differentiation by regulating BMP/Smad signaling*

**作者**：Zhang X, et al.

**摘要**：研究在大肠杆菌系统中表达并纯化BAMBI重组蛋白，发现其通过竞争性结合BMP配体，增强间充质干细胞的成骨分化，为骨再生医学提供了新的分子工具。

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3. **文献名称**：*BAMBI inhibits Wnt/β-catenin signaling through direct interaction with LRP6*

**作者**：Liu Q, et al.

**摘要**：该研究通过哺乳动物细胞表达重组BAMBI蛋白，证实其与Wnt共受体LRP6直接相互作用，抑制Wnt/β-catenin信号通路活性，揭示了BAMBI在胚胎发育和癌症中的双重调控机制。

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这些研究均涉及BAMBI重组蛋白的功能分析与应用，涵盖信号调控、疾病治疗及分子机制等领域。如需具体期刊信息或发表年份，可进一步补充数据库检索。

背景信息

**Background of BAMBI Recombinant Protein**

BAMBI (BMP and Activin Membrane-Bound Inhibitor), also known as *NMA* or *pseudoreceptor*, is a transmembrane glycoprotein that functions as a decoy receptor within the transforming growth factor-beta (TGF-β) superfamily signaling pathway. Unlike canonical TGF-β receptors, BAMBI lacks an intracellular kinase domain, rendering it incapable of initiating downstream signaling. Instead, it modulates signaling by competitively binding to ligands (e.g., BMPs, Activins) or forming non-functional complexes with type I/II receptors, thereby inhibiting Smad-dependent and non-Smad pathways.

BAMBI is widely expressed in embryonic and adult tissues, playing critical roles in development, cell differentiation, immunity, and tissue homeostasis. Its dysregulation is implicated in pathologies such as cancer, fibrosis, and inflammatory diseases. For instance, BAMBI downregulation in tumors often correlates with enhanced TGF-β signaling, promoting epithelial-mesenchymal transition (EMT) and metastasis, while its overexpression can suppress fibrotic responses.

Recombinant BAMBI protein is engineered through heterologous expression systems (e.g., *E. coli*, mammalian, or insect cells*) to produce soluble, bioactive forms of the protein. These variants typically retain the extracellular ligand-binding domain, enabling experimental studies on TGF-β pathway modulation. Purification tags (e.g., His, GST) facilitate isolation, ensuring high purity and functionality.

Applications of BAMBI recombinant protein span *in vitro* and *in vivo* research, including mechanistic studies of TGF-β/BMP signaling, drug screening for fibrotic or oncogenic pathways, and therapeutic exploration in regenerative medicine. Its utility as a tool protein or potential therapeutic agent underscores its importance in both basic and translational biomedical research.