纯度 | >95%SDS-PAGE. |
种属 | Human |
靶点 | ZNF70 |
Uniprot No | Q9UC06 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-446aa |
氨基酸序列 | MEVPPATKFGETFAFENRLESQQGLFPGEDLGDPFLQERGLEQMAVIYKE IPLGEQDEENDDYEGNFSLCSSPVQHQSIPPGTRPQDDELFGQTFLQKSD LSMCQIIHSEEPSPCDCAETDRGDSGPNAPHRTPQPAKPYACRECGKAFS QSSHLLRHLVIHTGEKPYECCECGKAFSQSSHLLRHQIIHTGEKPYECRE CGKAFRQSSALTQHQKIHTGKRPYECRECGKDFSRSSSLRKHERIHTGER PYQCKECGKSFNQSSGLSQHRKIHTLKKPHECDLCGKAFCHRSHLIRHQR IHTGKKPYKCDECGKAFSQSSNLIEHRKTHTGEKPYKCQKCGKAFSQSSS LIEHQRIHTGEKPYECCQCGKAFCHSSALIQHQRIHTGKKPYTCECGKAF RHRSALIEHYKTHTREKPYVCNLCGKSFRGSSHLIRHQKIHSGEKL |
预测分子量 | 52 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZNF70重组蛋白的假设参考文献示例(注:以下内容为虚构,仅用于演示格式):
1. **文献名称**: "Cloning, Expression, and Purification of Recombinant ZNF70 Protein in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了ZNF70基因的克隆及在大肠杆菌中的高效表达与纯化,优化了重组蛋白的可溶性,并验证了其锌指结构域的正确折叠。
2. **文献名称**: "Functional Characterization of ZNF70 as a Transcriptional Repressor in Human Cells"
**作者**: Smith J, Brown K.
**摘要**: 通过重组ZNF70蛋白的体外实验,发现其特异性结合特定DNA序列,并招募组蛋白去乙酰化酶(HDAC)复合物,抑制靶基因的转录活性。
3. **文献名称**: "ZNF70 Interacts with Viral Proteins and Modulates Innate Immune Responses"
**作者**: Chen R, et al.
**摘要**: 研究利用重组ZNF70蛋白进行互作组分析,揭示其与HIV-1 Tat蛋白的相互作用,可能通过干扰病毒复制通路参与宿主抗病毒反应。
4. **文献名称**: "Structural Insights into the DNA-Binding Mechanism of ZNF70 via Cryo-EM Analysis"
**作者**: Patel S, et al.
**摘要**: 利用冷冻电镜解析了重组ZNF70蛋白与DNA复合物的三维结构,阐明了其锌指模块与靶序列结合的关键氨基酸残基。
(注:以上文献为模拟生成,如需真实文献请通过PubMed或Google Scholar检索。)
ZNF70 is a member of the zinc finger protein (ZFP) family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that mediate sequence-specific DNA binding. As a transcription factor, it plays roles in gene regulation, particularly in processes like cell differentiation, proliferation, and stress response. The ZNF70 gene is located on human chromosome 22q13.1 and encodes a protein containing multiple tandem zinc finger motifs, enabling interaction with specific DNA sequences to modulate transcriptional activity. Recombinant ZNF70 protein is typically engineered for in vitro studies to elucidate its molecular functions, including DNA-binding specificity, protein-protein interactions, and regulatory mechanisms.
Research on ZNF70 has linked it to immune responses and viral defense mechanisms. For example, it may act as a restriction factor against certain viruses by binding viral DNA or RNA, though its precise antiviral mechanisms remain under investigation. Additionally, ZNF70 is hypothesized to participate in epigenetic regulation by recruiting chromatin-modifying complexes to target gene loci, influencing chromatin structure and gene expression patterns.
The production of recombinant ZNF70 often involves expression systems like Escherichia coli or mammalian cells, followed by affinity purification using tags such as His or FLAG. Studies utilizing recombinant ZNF70 have employed techniques like electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and CRISPR/Cas9-based gene editing to explore its biological roles. While its full physiological significance is not yet fully understood, ZNF70 represents a promising target for understanding transcriptional regulation and developing therapeutic strategies for diseases involving dysregulated gene expression, including cancers and viral infections. Further research is needed to map its target genes and clarify its context-dependent functions in health and disease.
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