Recombinant Human ZNF70 protein

ZNF70 is a member of the zinc finger protein (ZFP) family, characterized by conserved Cys₂His₂ (C2H2) zinc finger domains that mediate sequence-specific DNA binding. As a transcription factor, it plays roles in gene regulation, particularly in processes like cell differentiation, proliferation, and stress response. The ZNF70 gene is located on human chromosome 22q13.1 and encodes a protein containing multiple tandem zinc finger motifs, enabling interaction with specific DNA sequences to modulate transcriptional activity. Recombinant ZNF70 protein is typically engineered for in vitro studies to elucidate its molecular functions, including DNA-binding specificity, protein-protein interactions, and regulatory mechanisms.

Research on ZNF70 has linked it to immune responses and viral defense mechanisms. For example, it may act as a restriction factor against certain viruses by binding viral DNA or RNA, though its precise antiviral mechanisms remain under investigation. Additionally, ZNF70 is hypothesized to participate in epigenetic regulation by recruiting chromatin-modifying complexes to target gene loci, influencing chromatin structure and gene expression patterns.

The production of recombinant ZNF70 often involves expression systems like Escherichia coli or mammalian cells, followed by affinity purification using tags such as His or FLAG. Studies utilizing recombinant ZNF70 have employed techniques like electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), and CRISPR/Cas9-based gene editing to explore its biological roles. While its full physiological significance is not yet fully understood, ZNF70 represents a promising target for understanding transcriptional regulation and developing therapeutic strategies for diseases involving dysregulated gene expression, including cancers and viral infections. Further research is needed to map its target genes and clarify its context-dependent functions in health and disease.