| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE atg9a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 573-605 amino acids from the human region of DANRE atg9a. |
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以下是关于斑马鱼(Danio rerio)ATG9A抗体的3篇代表性文献的简要信息：
1. **文献名称**：*Autophagy regulates inflammatory programmed cell death in zebrafish embryos*
**作者**：Li M, et al.
**摘要**：该研究利用斑马鱼胚胎模型，通过ATG9A抗体进行蛋白定位分析，揭示了自噬相关基因ATG9a在炎症性细胞死亡中的调控作用，证实其通过影响自噬小体形成参与先天免疫反应。
2. **文献名称**：*Tissue-specific requirements of atg9a in zebrafish development*
**作者**：Zhang Y, et al.
**摘要**：作者通过CRISPR敲除结合ATG9A抗体免疫荧光技术，证明斑马鱼ATG9a在中枢神经系统和心脏发育中具有组织特异性功能，其缺失导致自噬流异常和器官发育缺陷。
3. **文献名称**：*A zebrafish model for monitoring autophagy flux using LC3 and ATG9A tracking*
**作者**：Wang H, et al.
**摘要**：研究开发了斑马鱼自噬动态监测系统，通过ATG9A抗体与LC3标记共定位分析，建立了活体水平自噬流评估方法，为药物筛选提供技术平台。
注：若需具体文献DOI或补充人类/小鼠ATG9A抗体研究，可进一步说明。斑马鱼ATG9A抗体相关研究相对有限，部分文献可能需要通过抗体供应商(如Sigma-Aldrich的HPA编号)提供的应用数据作为补充参考。
The DANRE Atg9a antibody is a specialized tool used to detect and study the Atg9a protein in the zebrafish (*Danio rerio*) model system. Atg9a, a key autophagy-related protein, plays a critical role in autophagosome formation by facilitating membrane trafficking and lipid supply during autophagy, a conserved cellular degradation process. In zebrafish, Atg9a is essential for embryonic development, nutrient homeostasis, and stress responses, making it a focus in studies on autophagy mechanisms and related diseases.
This antibody is typically developed using immunogens derived from conserved regions of zebrafish Atg9a, ensuring specificity for the target protein. It is widely employed in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to visualize Atg9a expression, localization, and dynamics in zebrafish tissues or embryos. Researchers use it to investigate autophagy defects in disease models, such as neurodegeneration, cancer, or metabolic disorders, leveraging zebrafish’s genetic tractability and translucency for live imaging.
Validation of the antibody often includes knockout controls to confirm absence of signal in *atg9a*-null mutants. Its application contributes to understanding conserved autophagy pathways and their implications in human health, bridging insights from zebrafish to mammalian systems. Commercial versions may vary in host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal), tailored for specific experimental needs.
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