Rabbit Polyclonal (DANRE)usp13 Antibody

中文名： (DANRE) usp13抗体
别名： Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, usp13

货号: IPDX32936

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,zebrafish,
IF	咨询技术	Human,Mouse,zebrafish,
IHC	咨询技术	Human,Mouse,zebrafish,
ICC	技术咨询	Human,Mouse,zebrafish,
FCM	咨询技术	Human,Mouse,zebrafish,
Elisa	咨询技术	Human,Mouse,zebrafish,

产品详情

Aliases	Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, usp13
Entrez GeneID	558011
WB Predicted band size	97.5kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,zebrafish,
Immunogen	This DANRE usp13 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 387-420 amino acids from the Central region of DANRE usp13.

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Western blot analysis of lysates from zebra fish muscle, Zebrafish cell line (from left to right), using (DANRE) usp13 Antibody (Center)(Cat. #P32936). P32936 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

参考文献

以下是关于斑马鱼(Danio rerio)USP13抗体的示例参考文献。由于该领域的研究较为专精，实际文献可能有限，以下内容为模拟示例，供参考：

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1. **"Characterization of USP13 in Zebrafish Embryonic Development"**

*Authors: Smith J, Lee R, Tanaka M*

摘要：本研究利用斑马鱼模型探讨USP13在胚胎发育中的作用。作者开发了一种针对斑马鱼USP13的特异性抗体，并通过免疫印迹和免疫荧光证实其在早期胚胎中的表达模式，提示USP13可能参与神经管形成。

2. **"Ubiquitin-Specific Protease 13 (USP13) Regulates DNA Repair in Danio rerio"**

*Authors: Chen L, Wang H, González F*

摘要：文章报道了斑马鱼USP13在DNA损伤修复中的功能。研究使用定制USP13抗体进行蛋白定位，发现其在体节细胞核内富集，并通过敲除实验证明USP13缺失导致修复机制缺陷。

3. **"Development of a Polyclonal Antibody Against Zebrafish USP13 and Its Application in Toxicity Studies"**

*Authors: Kim S, Patel R, O'Connor T*

摘要：本研究描述了斑马鱼USP13多克隆抗体的制备与验证。该抗体成功用于检测环境毒素暴露下USP13蛋白水平的变化，提示其作为生物标志物的潜力。

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**注意**：以上文献为示例，实际研究中可能需要通过学术数据库(如PubMed、Web of Science)以关键词“USP13 zebrafish antibody”或“Danio rerio USP13”进一步检索最新成果。若未找到直接结果，可考虑引用哺乳动物USP13抗体研究并提及其在斑马鱼中的潜在应用。

背景信息

The DANRE USP13 antibody is a research tool designed to target the ubiquitin-specific protease 13 (USP13) in the model organism *Danio rerio* (zebrafish). USP13 belongs to the deubiquitinating enzyme (DUB) family, which regulates protein stability and cellular processes by removing ubiquitin chains from substrate proteins. In zebrafish, USP13 is implicated in developmental pathways, stress responses, and disease-related mechanisms, such as protein quality control, DNA repair, and autophagy. Studies using this antibody aim to elucidate USP13's role in zebrafish physiology, particularly in embryogenesis, organogenesis, and disease modeling (e.g., cancer or neurodegenerative disorders).

The antibody is typically validated for specificity and efficacy in applications like Western blotting, immunohistochemistry, or immunofluorescence to detect endogenous USP13 expression. Its development often involves immunizing hosts (e.g., rabbits or mice) with zebrafish USP13-derived antigens, followed by affinity purification. Researchers utilize this tool to investigate conserved USP13 functions across vertebrates, leveraging zebrafish advantages like genetic tractability and transparent embryos.

Current research focuses on USP13's interaction with key signaling pathways (e.g., p53. NF-κB) and its potential as a therapeutic target. The DANRE USP13 antibody thus serves as a critical reagent for dissecting deubiquitination mechanisms in developmental and pathological contexts.