Recombinant Human STX7 protein

中文名： 重组人STX7蛋白
别名： STX7；Syntaxin-7

货号: PA1000-3996

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	STX7
Uniprot No	O15400
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	2-238aa
氨基酸序列	SYTPGVGGDP AQLAQRISSN IQKITQCSVE IQRTLNQLGT PQDSPELRQQ LQQKQQYTNQ LAKETDKYIK EFGSLPTTPS EQRQRKIQKD RLVAEFTTSL TNFQKVQRQA AEREKEFVAR VRASSRVSGS FPEDSSKERN LVSWESQTQP QVQVQDEEIT EDDLRLIHER ESSIRQLEAD IMDINEIFKD LGMMIHEQGD VIDSIEANVE NAEVHVQQAN QQLSRAADYQ RKSRKTL
预测分子量	30 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于STX7重组蛋白的3篇参考文献(均为虚拟示例，供参考)：

1. **文献名称**：*Functional Characterization of Recombinant STX7 in Lysosomal Membrane Fusion*

**作者**：Smith A, et al.

**摘要**：研究通过大肠杆菌表达系统成功纯化STX7重组蛋白，并验证其在体外膜融合实验中的功能，揭示STX7与VAMP8的相互作用对溶酶体成熟的关键作用。

2. **文献名称**：*Structural Insights into STX7 SNARE Complex Assembly*

**作者**：Lee C, et al.

**摘要**：利用重组STX7蛋白进行晶体结构分析，阐明其与SNAP-25及VAMP7形成四螺旋束的分子机制，为膜融合动态过程提供结构基础。

3. **文献名称**：*STX7 Recombinant Protein Modulates Autophagosome-Lysosome Fusion in Neurodegenerative Models*

**作者**：Zhang Y, et al.

**摘要**：通过体外递送重组STX7蛋白，证明其增强自噬体-溶酶体融合的能力，改善阿尔茨海默病细胞模型中蛋白聚集体的清除效率。

(注：以上为模拟内容，实际文献需通过学术数据库检索。)

背景信息

**Background of STX7 Recombinant Protein**

Syntaxin 7 (STX7) is a member of the syntaxin family of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, which play critical roles in intracellular membrane fusion events. STX7 is primarily localized to late endosomes and lysosomes, where it facilitates vesicular trafficking and organelle maturation. As a key component of the endolysosomal system, it participates in processes like endosome-to-lysosome transport, phagosome maturation, and autophagosome-lysosome fusion, essential for cellular homeostasis, nutrient sensing, and pathogen degradation.

The recombinant STX7 protein is engineered to enable detailed biochemical and functional studies. Produced using expression systems like *E. coli* or mammalian cells, it retains the native protein's structural and functional properties, including its SNARE motif and transmembrane domain. Researchers utilize recombinant STX7 to investigate its interactions with partner SNAREs (e.g., VAMP7/VAMP8 and SNAP-29), regulatory proteins (e.g., Rab GTPases), and its role in membrane docking/fusion mechanisms.

STX7 dysregulation has been linked to diseases such as cancer, neurodegenerative disorders, and lysosomal storage diseases. For example, altered STX7 expression affects lysosomal function, contributing to impaired autophagy in conditions like Alzheimer’s disease. Recombinant STX7 serves as a tool for drug discovery, enabling high-throughput screening of compounds targeting endolysosomal pathways. It also aids in antibody development for diagnostic assays and mechanistic studies of intracellular trafficking defects.

Overall, STX7 recombinant protein bridges molecular research and therapeutic exploration, offering insights into cellular logistics and disease mechanisms tied to membrane dynamics.