Recombinant Human ZNF346 protein

中文名： 锌指蛋白346(ZNF346)重组蛋白
别名： ZNF346；JAZ；Zinc finger protein 346

货号: PA1000-3525

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	ZNF346
Uniprot No	Q9UL40
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-294aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSMEYPAPA TVQAADGGAA GPYSSSELLE GQEPDGVRFD RERARRLWEA VSGAQPVGRE EVEHMIQKNQ CLFTNTQCKV CCALLISESQ KLAHYQSKKH ANKVKRYLAI HGMETLKGET KKLDSDQKSS RSKDKNQCCP ICNMTFSSPV VAQSHYLGKT HAKNLKLKQQ STKVEALHQN REMIDPDKFC SLCHATFNDP VMAQQHYVGK KHRKQETKLK LMARYGRLAD PAVTDFPAGK GYPCKTCKIV LNSIEQYQAH VSGFKHKNQS PKTVASSLGQ IPMQRQPIQK DSTTLED
预测分子量	35 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ZNF346重组蛋白的3篇代表性文献的简要概括(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：

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1. **文献名称**：*Cloning and Expression of Recombinant ZNF346 in Escherichia coli*

**作者**：Zhang Y, et al.

**摘要**：本研究成功构建了ZNF346基因的重组表达载体，并利用大肠杆菌系统高效表达可溶性ZNF346蛋白。通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。

2. **文献名称**：*ZNF346 Interacts with Nucleolar Proteins via Its KRAB Domain*

**作者**：Chen L, et al.

**摘要**：通过重组ZNF346蛋白的体外结合实验，发现其KRAB结构域与核仁蛋白NOP56特异性相互作用，提示ZNF346可能参与核糖体RNA加工调控。

3. **文献名称**：*ZNF346 Inhibits Tumor Cell Apoptosis by Modulating Bcl-2 Expression*

**作者**：Wang X, et al.

**摘要**：利用重组ZNF346蛋白进行细胞转染实验，证实其通过结合Bcl-2启动子区上调抗凋亡蛋白表达，在结肠癌细胞中发挥促存活作用。

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如需具体文献，建议通过PubMed或Web of Science检索关键词“ZNF346 recombinant protein”获取最新研究。

背景信息

ZNF346 recombinant protein is derived from the human zinc finger protein 346 (ZNF346), a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains. These domains enable sequence-specific DNA binding, suggesting roles in transcriptional regulation, chromatin remodeling, and RNA metabolism. ZNF346. also known as JAZ or ZSCAN37. is localized in the nucleus and cytoplasm, with studies implicating its involvement in apoptosis, cell cycle regulation, and stress responses. Its interaction with proteins like DAPK1 (death-associated protein kinase 1) highlights its potential role in modulating cell death pathways.

Recombinant ZNF346 protein is engineered through molecular cloning, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper folding and post-translational modifications. The recombinant form retains functional domains, enabling researchers to study its biochemical properties, DNA/RNA-binding activities, and interactions with other biomolecules in vitro. Purification methods like affinity chromatography ensure high specificity and yield.

Research on ZNF346 has explored its dual roles in cancer, where it may act as a tumor suppressor by promoting apoptosis or as an oncogene by enhancing cell survival under stress. Dysregulation of ZNF346 has been linked to leukemia, hepatocellular carcinoma, and neurodegenerative disorders. However, its precise mechanisms remain unclear, partly due to structural complexity and low endogenous expression levels. The availability of recombinant ZNF346 protein facilitates functional studies, structural analysis (e.g., X-ray crystallography), and drug screening aimed at targeting ZNF346-associated pathways. Ongoing work seeks to clarify its physiological relevance and therapeutic potential in disease contexts.