Recombinant Human TTC32 protein

**Background of TTC32 Recombinant Protein**

TTC32 (Tetratricopeptide Repeat Domain 32) is a member of the TTC protein family characterized by conserved tetratricopeptide repeat (TPR) motifs, which are structural elements involved in mediating protein-protein interactions. The human *TTC32* gene, located on chromosome 7. encodes a 32 kDa protein predominantly expressed in tissues such as the testis, brain, and liver. While its precise biological role remains under investigation, TTC32 is hypothesized to participate in cellular processes like protein complex assembly, intracellular trafficking, or modulation of enzymatic activity, leveraging its TPR domains to recruit partner molecules.

Recombinant TTC32 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. This enables detailed studies of its structure, interactome, and mechanistic contributions to physiology and disease. Emerging evidence links TTC32 dysfunction to human pathologies. For instance, it interacts with components of the 26S proteasome (e.g., PSMD2 and PSMD4), suggesting a role in protein degradation regulation. Mutations in *TTC32* have also been associated with male infertility due to impaired spermatogenesis, possibly through disrupted interactions with germ cell-specific proteins. Additionally, altered TTC32 expression has been observed in certain cancers, though its pro- or anti-tumorigenic effects remain unclear.

The availability of recombinant TTC32 facilitates antibody development, *in vitro* binding assays, and structural analyses (e.g., X-ray crystallography). These tools are critical for elucidating its molecular functions and validating its potential as a therapeutic target or biomarker. Ongoing research aims to clarify its physiological relevance and explore its interplay with signaling pathways, such as ubiquitin-proteasome or stress-response systems. Understanding TTC32's role may provide insights into disorders linked to protein homeostasis or cellular trafficking defects.