Recombinant Human ATP5C1 protein

中文名： ATP合成酶γ亚基,线粒体(ATP5C1)重组蛋白
别名： ATP5C1；ATP5C；ATP5C1；ATP5CL1；ATP synthase subunit gamma, mitochondrial

货号: PA1000-273DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85% (SDS-PAGE)
种属	Human
靶点	ATP5C1
Uniprot No	P36542
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	26-298aa
氨基酸序列	ATLKD ITRRLKSIKN IQKITKSMKM VAAAKYARAE RELKPARIYG LGSLALYEKA DIKGPEDKKK HLLIGVSSDR GLCGAIHSSI AKQMKSEVAT LTAAGKEVML VGIGDKIRGI LYRTHSDQFL VAFKEVGRKP PTFGDASVIA LELLNSGYEF DEGSIIFNKF RSVISYKTEE KPIFSLNTVA SADSMSIYDD IDADVLQNYQ EYNLANIIYY SLKESTTSEQ SARMTAMDNA SKNASEMIDK LTLTFNRTRQ AVITKELIEI ISGAAALD
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ATP5C1重组蛋白的参考文献示例(建议通过PubMed或Google Scholar核实最新文献)：

1. **文献名称**：Crystal structure of the human mitochondrial ATP synthase γ-subunit (ATP5C1) reveals conserved structural motifs

**作者**：Walker JE, et al.

**摘要**：解析了人源ATP5C1重组蛋白的晶体结构，揭示了其与ATP合酶复合物结合的构象变化机制，为线粒体能量代谢研究提供结构基础。

2. **文献名称**：Functional characterization of ATP5C1 mutations in mitochondrial encephalopathy using recombinant protein models

**作者**：Zeviani M, et al.

**摘要**：通过重组表达ATP5C1突变体，证明其导致ATP水解活性异常，与线粒体脑肌病的氧化磷酸化缺陷相关。

3. **文献名称**：High-yield expression and purification of recombinant ATP5C1 in Escherichia coli for antibody production

**作者**：Racker E, et al.

**摘要**：开发了基于大肠杆菌的ATP5C1重组蛋白高效表达纯化系统，用于生成特异性抗体，支持后续功能研究。

4. **文献名称**：ATP5C1 overexpression promotes cancer cell proliferation via altered mitochondrial bioenergetics

**作者**：Gottlieb E, et al.

**摘要**：发现ATP5C1重组蛋白在肿瘤细胞中异常高表达，通过增强线粒体膜电位促进肿瘤代谢重编程。

注：以上为模拟示例，实际文献需通过学术数据库检索。推荐使用关键词“ATP5C1 recombinant protein”、“ATP synthase gamma subunit”结合研究领域(如结构/疾病/表达纯化)筛选文献。

背景信息

ATP5C1. also known as the gamma subunit of mitochondrial ATP synthase (Complex V), is a critical component of the oxidative phosphorylation (OXPHOS) machinery responsible for cellular energy production. This nuclear-encoded protein localizes to the mitochondrial inner membrane, where it forms part of the catalytic F1 domain of ATP synthase. The enzyme complex couples proton gradient-driven rotation of its transmembrane FO sector with ATP synthesis in the F1 domain through a mechanism involving conformational changes in the gamma subunit. As the central rotor stalk component, ATP5C1 plays a structural and functional role in coordinating energy transfer between FO and F1 domains.

Recombinant ATP5C1 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and structural studies. Its production enables detailed investigation of ATP synthase assembly, enzyme kinetics, and proton transport mechanisms. Researchers utilize purified ATP5C1 to study mutations affecting mitochondrial function, particularly those linked to neuromuscular disorders and metabolic diseases. For instance, specific ATP5C1 variants have been associated with mitochondrial encephalopathy and ATP synthase deficiencies characterized by impaired cellular energy metabolism.

Recent studies highlight ATP5C1's potential role in cancer progression, as many tumors show altered ATP synthase expression patterns. The recombinant protein serves as a tool for developing inhibitors targeting cancer-specific ATP synthase isoforms. Additionally, ATP5C1 has been implicated in neurodegenerative conditions like Alzheimer's disease, where mitochondrial dysfunction contributes to pathogenesis. Structural analyses using recombinant protein help map interaction interfaces with regulatory proteins like IF1 (ATPase inhibitory factor 1), providing insights into metabolic regulation under hypoxic conditions. Current research focuses on understanding post-translational modifications of ATP5C1 and their impact on cellular adaptation to energy stress.