| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRM2 |
| Uniprot No | P31350 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-389aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENTPPALSGT RVLASKTARR IFQEPTEPKT KAAAPGVEDEPLLRENPRRF VIFPIEYHDI WQMYKKAEAS FWTAEEVDLS KDIQHWESLK PEERYFISHV LAFFAASDGI VNENLVERFS QEVQITEARC FYGFQIAMEN IHSEMYSLLI DTYIKDPKER EFLFNAIETM PCVKKKADWA LRWIGDKEAT YGERVVAFAA VEGIFFSGSF ASIFWLKKRGLMPGLTFSNE LISRDEGLHC DFACLMFKHL VHKPSEERVR EIIINAVRIE QEFLTEALPV KLIGMNCTLM KQYIEFVADR LMLELGFSKV FRVENPFDFM ENISLEGKTN FFEKRVGEYQ RMGVMSSPTE NSFTLDADF |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RRM2重组蛋白的3篇参考文献示例(注：文献信息为模拟内容，实际引用时请核对准确性)：
1. **《RRM2 promotes DNA damage response through modulating p53 activity》**
- 作者：Fan H, et al.
- 摘要：研究利用重组RRM2蛋白，揭示了其通过调控p53磷酸化参与DNA损伤修复的分子机制，为癌症治疗提供了新靶点。
2. **《Expression and purification of recombinant human RRM2 for functional studies》**
- 作者：Zhang Y, et al.
- 摘要：报道了人源RRM2重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其在体外催化核糖核苷酸还原的活性。
3. **《Targeting RRM2 overexpression enhances chemosensitivity in lung cancer》**
- 作者：Wang L, et al.
- 摘要：通过重组RRM2蛋白的体外实验，证明抑制RRM2可增强肺癌细胞对吉西他滨的敏感性，提示其作为化疗增敏剂的潜力。
**注意**：以上文献信息为示例，实际研究中建议通过PubMed或Web of Science等平台检索最新文献，并核实作者、标题及摘要的准确性。
**Background of Recombinant RRM2 Protein**
Ribonucleotide reductase regulatory subunit M2 (RRM2) is a critical component of the ribonucleotide reductase (RNR) enzyme complex, which plays an essential role in DNA synthesis and repair by catalyzing the conversion of ribonucleotides to deoxyribonucleotides. As a regulatory subunit, RRM2 works in conjunction with the catalytic subunit RRM1 to maintain optimal dNTP pool balance, ensuring genomic stability and cell cycle progression. Its expression is tightly regulated during the cell cycle, peaking in the S phase to meet the high demand for DNA replication. Dysregulation of RRM2 has been linked to various cancers, where its overexpression is associated with tumor proliferation, metastasis, and resistance to chemotherapy.
Recombinant RRM2 protein is produced using genetic engineering techniques, typically through expression in bacterial or mammalian systems, followed by purification to ensure high specificity and activity. This engineered protein retains the functional properties of native RRM2. enabling researchers to study its biochemical interactions, enzymatic mechanisms, and regulatory roles in vitro. Recombinant RRM2 is widely utilized in structural studies, enzyme activity assays, and screening for inhibitors that could target RNR as a therapeutic strategy. Additionally, it serves as a vital tool for investigating RRM2's interplay with other proteins, such as tumor suppressors or oncogenic signaling molecules, in pathways related to DNA damage response and cell survival.
The development of recombinant RRM2 has significantly advanced cancer research, particularly in understanding chemoresistance mechanisms and identifying novel anticancer agents. By modulating RRM2 activity, researchers aim to disrupt cancer cell proliferation while sparing normal cells, offering a potential avenue for precision therapies. Its role in maintaining genomic integrity also underscores its relevance in studying aging, neurodegenerative diseases, and other conditions linked to DNA repair defects. Overall, recombinant RRM2 remains a cornerstone protein in both basic and translational biomedical research.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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