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Recombinant Human RNF181 protein

  • 中文名: 环指蛋白181(RNF181)重组蛋白
  • 别    名: RNF181;E3 ubiquitin-protein ligase RNF181
货号: PA1000-2739
Price: ¥询价
数量:
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产品详情

纯度>95%SDS-PAGE.
种属Human
靶点RNF181
Uniprot NoQ9P0P0
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-153aa
氨基酸序列MGSSHHHHHHSSGLVPRGSHMGSMASYFDEHDCEPSDPEQETRTNMLLEL ARSLFNRMDFEDLGLVVDWDHHLPPPAAKTVVENLPRTVIRGSQAELKCP VCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDDDTY EEHRRDKARKQQQQHRLENLHGAMYT
预测分子量20 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于RNF181重组蛋白的3篇参考文献及其摘要概括:

1. **"RNF181 modulates β3 integrin signaling in platelets by regulating the stability of a focal adhesion complex"**

- 作者:Vallet et al.

- 摘要:研究RNF181通过其E3泛素连接酶活性调控血小板中β3整合素信号通路,利用重组蛋白实验证实其与整合素复合物的相互作用,并影响血小板黏附和聚集功能。

2. **"RNF181 acts as a tumor suppressor in prostate cancer by destabilizing oncogenic AR-V7 protein"**

- 作者:Wang et al.

- 摘要:发现RNF181通过泛素化降解雄激素受体变异体AR-V7.抑制前列腺癌进展。研究中使用重组RNF181蛋白验证其直接结合并泛素化AR-V7的分子机制。

3. **"RNF181 promotes hepatocellular carcinoma progression by ubiquitinating and stabilizing YAP1"**

- 作者:Zhang et al.

- 摘要:揭示RNF181在肝细胞癌中通过泛素化修饰稳定YAP1蛋白,促进肿瘤生长和转移。重组RNF181蛋白体外实验证实其对YAP1的E3连接酶活性。

这些研究均涉及RNF181重组蛋白在功能验证中的应用,涵盖其在信号通路调控、癌症进展中的分子机制。如需具体文献来源,可进一步通过PubMed或期刊数据库查询完整信息。

背景信息

RNF181 (Ring Finger Protein 181) is a member of the RING finger protein family, characterized by a conserved zinc-binding RING domain that often confers E3 ubiquitin ligase activity. This protein is implicated in regulating ubiquitination, a post-translational modification critical for protein degradation, signaling, and cellular homeostasis. RNF181 is broadly expressed in human tissues, including the cardiovascular system, brain, and immune cells, suggesting diverse functional roles. Studies link it to cellular processes such as cell adhesion, proliferation, apoptosis, and inflammatory responses, though its precise molecular mechanisms remain under investigation.

Research highlights RNF181's interaction with integrins, key mediators of cell-extracellular matrix communication. By modulating integrin signaling pathways, RNF181 may influence platelet activation, vascular function, and immune cell trafficking. Its E3 ligase activity likely targets specific substrates for ubiquitination, potentially regulating their stability or interactions. Dysregulation of RNF181 has been associated with pathological conditions, including atherosclerosis, thrombosis, and cancer metastasis, positioning it as a potential therapeutic target or biomarker.

Recombinant RNF181 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. It serves as a vital tool for *in vitro* studies to elucidate RNF181's structure-function relationships, substrate specificity, and interaction networks. Current research aims to clarify its role in disease contexts and explore its utility in drug discovery or diagnostic applications. Despite progress, further work is needed to fully unravel its biological significance and translational potential.

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