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Recombinant Human ASPHD1 protein

  • 中文名: 天冬氨酸β羟化酶D1(ASPHD1)重组蛋白
  • 别    名: ASPHD1;Aspartate beta-hydroxylase domain-containing protein 1
货号: PA1000-253DB
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**Background of ASPH Recombinant Protein**

Aspartate β-hydroxylase (ASPH) is a transmembrane enzyme that catalyzes the post-translational hydroxylation of specific aspartyl and asparaginyl residues in epidermal growth factor-like domains (EGFDs) of proteins. Initially identified for its role in calcium-binding protein modifications, ASPH has gained significant attention due to its overexpression in various cancers, including hepatocellular carcinoma, breast cancer, and pancreatic cancer. This enzyme is implicated in promoting tumor progression, metastasis, and angiogenesis by activating signaling pathways such as Notch, Wnt/β-catenin, and PI3K/Akt, which are critical for cell proliferation, survival, and invasion.

Recombinant ASPH proteins are engineered to study its biochemical functions, structure, and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), these proteins retain key domains, such as the catalytic β-hydroxylase domain, enabling researchers to investigate enzyme kinetics, substrate specificity, and inhibitor screening. Purification techniques like affinity chromatography ensure high-purity yields for functional assays.

ASPH is considered a promising therapeutic target, driving the development of small-molecule inhibitors, monoclonal antibodies, and immunotherapies. Recombinant ASPH also aids in diagnostic applications, as elevated ASPH levels correlate with poor prognosis in cancer patients. Structural studies using recombinant variants have elucidated mechanisms of substrate recognition and catalysis, guiding rational drug design.

In summary, ASPH recombinant proteins serve as vital tools for unraveling the enzyme’s oncogenic roles, validating therapeutic strategies, and advancing translational research in oncology and beyond.

参考文献

以下是关于ASPHD1重组蛋白的模拟参考文献示例(仅供参考,实际文献请通过学术数据库查询):

1. **文献名称**:*Cloning and Functional Characterization of ASPHD1 as a Novel Histone Demethylase*

**作者**:Zhang Y, et al.

**摘要**:研究报道了ASPHD1重组蛋白的克隆与酶活性分析,发现其JmjC结构域具有组蛋白去甲基化酶活性,可能参与表观遗传调控。

2. **文献名称**:*ASPHD1 Expression Correlates with Tumor Metastasis in Hepatocellular Carcinoma*

**作者**:Li H, Wang X.

**摘要**:通过重组ASPHD1蛋白的功能实验,揭示了其在肝癌细胞侵袭转移中的作用,可能通过调控Wnt/β-catenin通路促进肿瘤进展。

3. **文献名称**:*Structural Insights into ASPHD1’s Catalytic Domain by X-ray Crystallography*

**作者**:Smith J, et al.

**摘要**:解析了ASPHD1重组蛋白的晶体结构,阐明了其活性位点的关键氨基酸残基,为靶向药物设计提供了结构基础。

4. **文献名称**:*ASPHD1 Knockout Mice Exhibit Impaired Neuronal Differentiation*

**作者**:Chen L, et al.

**摘要**:利用重组ASPHD1蛋白进行体外拯救实验,证明其缺失导致小鼠神经干细胞分化障碍,提示其在神经发育中的潜在功能。

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**备注**:以上为模拟文献,真实研究需通过PubMed、Google Scholar等平台检索关键词“ASPHD1 recombinant protein”或“ASPHD1 function”获取。

背景信息

ASPHD1 (Aspartate Beta-Hydroxylase Domain-Containing Protein 1) is a less-studied member of the α-ketoglutarate-dependent dioxygenase family, which is implicated in post-translational modifications and epigenetic regulation. The protein contains a conserved aspartyl β-hydroxylase domain, structurally similar to enzymes that catalyze hydroxylation reactions, though its precise biochemical activity remains under investigation. ASPHD1 is encoded by the ASPHD1 gene located on human chromosome 16 and is expressed in various tissues, with notable presence in the brain, liver, and reproductive organs. Emerging evidence links ASPHD1 to cellular processes such as differentiation, apoptosis, and chromatin remodeling, potentially through interactions with histone-modifying complexes or hydroxylation of specific substrates.

Recombinant ASPHD1 protein is engineered for in vitro studies to elucidate its molecular functions. It is typically produced using heterologous expression systems like Escherichia coli or mammalian cell lines, followed by affinity chromatography purification (e.g., His-tag or GST-tag systems). The recombinant protein enables researchers to analyze enzymatic activity, substrate specificity, and structural features via techniques like crystallography or enzyme kinetics assays. Notably, ASPHD1 has been associated with human diseases, including neurodevelopmental disorders and cancers. For example, aberrant ASPHD1 expression is observed in glioblastoma and hepatocellular carcinoma, suggesting a role in tumor progression. In neurodegenerative contexts, it may influence pathways related to Alzheimer’s disease, though mechanistic insights are limited.

Current research leverages recombinant ASPHD1 to develop targeted inhibitors or probes, aiming to dissect its physiological relevance and therapeutic potential. Challenges include clarifying its endogenous substrates and reconciling conflicting data about its hydroxylation targets versus non-enzymatic roles. Overall, ASPHD1 recombinant protein serves as a critical tool for bridging gaps in understanding its contribution to epigenetics and disease.

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