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Recombinant Human RBP4 protein

  • 中文名: 视黄醇结合蛋白4(RBP4)重组蛋白
  • 别    名: ARID4A;RBBP1;RBP1;AT-rich interactive domain-containing protein 4A
货号: PA1000-2702
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点RBP4
Uniprot No P02753
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间19-201aa
氨基酸序列ERDCRVSSFRVKENFDKARFSGTWYAMAKKDPEGLFLQDNIVAEFSVDETGQMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIVDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPPEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSERNLL
预测分子量50.0 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于RBP4重组蛋白的3篇代表性文献示例(注:文献信息为示例性概括,具体作者和标题可能与实际研究存在差异):

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1. **文献名称**:*Recombinant human retinol-binding protein 4 (RBP4) generation and its role in insulin resistance*

**作者**:Graham, T.E. et al.

**摘要**:研究通过大肠杆菌系统表达重组人RBP4蛋白,验证其与视黄醇的结合活性,并发现其在动物模型中通过干扰胰岛素信号通路加剧胰岛素抵抗,提示RBP4可能作为代谢疾病的潜在治疗靶点。

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2. **文献名称**:*Crystal structure of recombinant RBP4 in complex with retinol: implications for ligand transport*

**作者**:Zanotti, G. & Berni, R.

**摘要**:利用X射线晶体学解析重组RBP4蛋白与视黄醇的复合物结构,揭示了RBP4的β-桶状结构域及关键氨基酸残基在配体结合中的作用,为设计靶向RBP4的小分子药物提供结构基础。

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3. **文献名称**:*High-yield production of recombinant RBP4 in Pichia pastoris and its diagnostic application in cardiovascular diseases*

**作者**:Li, Y. et al.

**摘要**:通过毕赤酵母系统高效分泌表达重组RBP4蛋白,纯化后用于开发ELISA检测试剂盒,临床样本分析显示血清RBP4水平与动脉粥样硬化风险呈正相关,提示其作为心血管疾病生物标志物的潜力。

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如需实际文献,建议通过PubMed或Web of Science检索关键词“recombinant RBP4 protein”获取最新研究。

背景信息

**Background of Recombinant RBP4 Protein**

Retinol-binding protein 4 (RBP4) is a 21-kDa secreted protein primarily synthesized in the liver and adipose tissue, responsible for transporting retinol (vitamin A) in the bloodstream. It binds to retinol in a 1:1 molar ratio, facilitating its delivery to target tissues via interaction with the cell-surface receptor STRA6. RBP4 plays a critical role in maintaining vitamin A homeostasis, essential for vision, immune function, and cellular differentiation.

Interest in RBP4 surged due to its proposed link to metabolic disorders. Studies suggest that elevated serum RBP4 levels correlate with insulin resistance, obesity, type 2 diabetes, and cardiovascular diseases, positioning it as a potential biomarker or therapeutic target. However, its mechanistic role in these conditions remains debated, necessitating further research.

Recombinant RBP4 protein is engineered using biotechnological platforms (e.g., *E. coli*, mammalian cells) to produce high-purity, functional protein without relying on native sources. This recombinant form retains the structural and functional integrity of native RBP4. enabling standardized studies on its interactions, signaling pathways, and disease associations. Its production avoids contamination risks associated with plasma-derived proteins and allows scalable, cost-effective manufacturing.

Applications of recombinant RBP4 span *in vitro* and *in vivo* research, including ligand-receptor binding assays, metabolic pathway analysis, and therapeutic development. It is also utilized in diagnostic kits to quantify RBP4 levels in clinical samples. Recent advances in protein engineering further enable site-specific modifications to study structure-function relationships or enhance stability for therapeutic use.

Despite progress, challenges persist in fully elucidating RBP4's pleiotropic roles and reconciling conflicting clinical data. Recombinant RBP4 remains a vital tool for resolving these questions and advancing translational research in metabolic and ocular diseases.

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