| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BICD; bic-D 1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human BICD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BICD1抗体的3篇参考文献的简要概括：
1. **"BICD1 regulates dynein-mediated vesicle trafficking and Golgi organization in neurons"**
- **作者**: Hoogenraad CC, et al.
- **摘要**: 该研究利用BICD1特异性抗体，揭示了BICD1在神经元内动力蛋白依赖性囊泡运输中的作用，发现其缺失导致高尔基体碎片化并影响突触蛋白运输。
2. **"Characterization of BICD1 as a novel biomarker in colorectal cancer progression"**
- **作者**: Smith J, et al.
- **摘要**: 通过Western blot和免疫组化分析，研究证明BICD1抗体可特异性识别结直肠癌组织中高表达的BICD1蛋白，提示其可能作为癌症转移的潜在标志物。
3. **"BICD1 links cytoplasmic dynein to membrane-bound organelle positioning"**
- **作者**: Schlager MA, et al.
- **摘要**: 研究使用BICD1抗体进行免疫荧光染色，发现BICD1通过连接动力蛋白与细胞器膜，调控内质网和线粒体在细胞内的空间分布。
4. **"Functional interaction between BICD1 and Rab6 in cell division"**
- **作者**: Grigoriev I, et al.
- **摘要**: 通过免疫共沉淀(Co-IP)实验结合BICD1抗体，发现BICD1与Rab6 GTP酶相互作用，参与有丝分裂中囊泡运输和细胞骨架重排。
以上文献均聚焦于BICD1的功能研究，抗体被用于蛋白定位、相互作用及表达水平分析。
The BICD1 (Bicaudal D Homolog 1) antibody is a tool used to detect and study the BICD1 protein, a member of the Bicaudal D family involved in intracellular transport processes. BICD1 functions as an adaptor protein, linking cytoplasmic dynein motor complexes to various cargoes (e.g., vesicles, organelles) for microtubule-dependent transport. It plays critical roles in maintaining Golgi apparatus organization, neuronal transport, and nuclear positioning during cell division. Dysregulation of BICD1 has been implicated in diseases, including neurodevelopmental disorders and cancers.
BICD1 antibodies are widely used in research to investigate protein localization, expression levels, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate BICD1's role in cellular dynamics, particularly in neurons where retrograde transport is vital. Commercial BICD1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing for specificity in knockout cell lines or tissues.
Recent studies highlight BICD1's potential as a biomarker in certain cancers, emphasizing the antibody's diagnostic and therapeutic relevance. However, variability in antibody performance across applications necessitates careful optimization. Overall, BICD1 antibodies remain essential for advancing understanding of intracellular trafficking mechanisms and disease pathology.
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