| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBXD3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human UBXN10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于UBXN10抗体的3篇参考文献的简要列举(注：部分内容基于领域内常见研究方向的模拟概括，作者和文献名为虚构示例)：
1. **文献名称**：UBXN10作为p97复合物调控因子在泛素化蛋白降解中的作用
**作者**：Tang W. et al.
**摘要**：本研究通过UBXN10特异性抗体验证了其与p97/VCP蛋白的相互作用，揭示了UBXN10通过调控p97复合物的ATP酶活性影响内质网相关降解(ERAD)通路，进而参与错误折叠蛋白的清除机制。
2. **文献名称**：UBXN10在结直肠癌中的表达缺失及其肿瘤抑制功能
**作者**：Zhang L. et al.
**摘要**：利用UBXN10抗体进行免疫组化分析，发现UBXN10在结直肠癌组织中表达显著下调。功能实验表明，UBXN10通过抑制NF-κB信号通路降低肿瘤细胞增殖和转移能力，提示其作为潜在生物标志物的价值。
3. **文献名称**：UBXN10抗体在细胞周期研究中的应用及机制探索
**作者**：Lee S. et al.
**摘要**：通过UBXN10抗体介导的免疫沉淀和RNA干扰技术，研究发现UBXN10与有丝分裂调控蛋白复合物结合，影响细胞周期G2/M期转换，其缺失导致染色体分离异常和基因组不稳定性。
4. **文献名称**：UBXN10蛋白结构解析及其抗体表位鉴定
**作者**：Smith J. et al.
**摘要**：本研究通过X射线晶体学解析UBXN10的UBX结构域，并利用单克隆抗体定位其抗原表位，为开发高特异性诊断工具及研究UBXN10功能提供了分子基础。
The UBXN10 antibody is a tool used to detect the UBXN10 protein, a member of the UBX domain-containing protein family. UBXN10. also known as UBXD10 or FAS-associated factor 2 (FAF2), plays roles in cellular processes such as endoplasmic reticulum-associated degradation (ERAD), lipid droplet regulation, and apoptosis. It interacts with key proteins like p97/VCP (valosin-containing protein), linking it to ubiquitin-proteasome pathways and protein quality control. UBXN10’s involvement in ERAD highlights its importance in clearing misfolded proteins, while its lipid droplet association suggests regulatory functions in lipid metabolism.
Antibodies targeting UBXN10 are typically developed in hosts like rabbits or mice, using immunogens derived from specific regions of the human UBXN10 sequence. These antibodies are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation, aiding in studies of UBXN10’s expression, localization, and interactions. Researchers employ UBXN10 antibodies to explore its roles in diseases, including cancers (e.g., breast, liver) and metabolic disorders, where dysregulation of protein degradation or lipid homeostasis is implicated. Specificity is often confirmed using knockout cell lines or siRNA-mediated knockdown.
UBXN10 antibodies have become critical in elucidating the protein’s molecular mechanisms, particularly its dual roles in stress response and cellular homeostasis. Ongoing research focuses on its potential as a therapeutic target or biomarker in pathologies linked to proteostasis imbalance.
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