| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RECQ3; RECQL2; RECQL3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WRN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WRN抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*WRN Protein Expression in Human Cancer Tissues: A Focus on Its Nuclear-Extranuclear Compartmentalization*
**作者**：Smith J, et al.
**摘要**：该研究利用特异性WRN抗体(克隆号：XX)对多种癌症组织进行免疫组化分析，发现WRN蛋白在结直肠癌和乳腺癌中显著高表达，且核内与胞质定位差异与患者预后相关，提示其亚细胞分布可能影响肿瘤进展。
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2. **文献名称**：*Antibody-Based Detection of Werner Syndrome Helicase (WRN) in Senescent Cells*
**作者**：Li H, et al.
**摘要**：通过Western blot和免疫荧光技术，验证了WRN抗体(货号：ABC123)在衰老细胞模型中的特异性。研究发现，WRN蛋白在复制性衰老细胞中表达降低，且与DNA损伤修复能力下降相关，为衰老机制研究提供了工具支持。
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3. **文献名称**：*Comparative Analysis of Commercial WRN Antibodies for Research Applications*
**作者**：Garcia R, et al.
**摘要**：系统比较了市售的5种WRN抗体(包括兔多抗和小鼠单抗)，评估其在免疫沉淀、染色质共沉淀(ChIP)等实验中的性能。结果显示，抗体XX在ChIP中特异性最佳，适用于研究WRN与端粒DNA的相互作用。
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(注：上述文献为虚拟示例，实际引用需根据真实研究调整。)
The Werner syndrome protein (WRN), encoded by the *WRN* gene, is a member of the RecQ helicase family critical for maintaining genomic stability. Mutations in *WRN* cause Werner syndrome, a rare autosomal recessive disorder characterized by premature aging, genomic instability, and increased cancer susceptibility. The WRN protein exhibits 3'→5' helicase and 3'→5' exonuclease activities, playing roles in DNA repair, replication, recombination, and telomere maintenance. It resolves DNA secondary structures, facilitates replication stress recovery, and interacts with key repair proteins like PARP1 and BRCA1.
WRN antibodies are essential tools for studying its expression, localization, and molecular functions. They enable detection of WRN in immunofluorescence, Western blotting, and immunohistochemistry, helping assess its role in aging-related pathways and cancer biology. Research shows WRN is a synthetic lethal target in microsatellite-unstable (MSI) cancers, where its inhibition selectively kills cancer cells. This has spurred interest in developing WRN-targeted therapies. Antibodies also aid in analyzing WRN deficiency phenotypes, such as replication fork stalling and chromosomal breaks. Commercially available WRN antibodies target specific epitopes, with validation in knockout controls ensuring specificity. Their applications extend to clinical research, including biomarker studies in Werner syndrome and cancer therapeutics development.
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