| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OOMD5; WEE1B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human WEE2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于WEE2抗体的3篇代表性文献的简要信息(注：文献为模拟示例，实际引用需核实)：
1. **文献名称**：*WEE2 kinase deficiency mitigates oocyte maturation via CDC25A regulation*
**作者**：Li, R.; et al.
**摘要**：本研究利用特异性WEE2抗体，通过免疫印迹和免疫荧光技术，发现WEE2在小鼠卵母细胞减数分裂中通过调控CDC25A磷酸化水平影响细胞周期进程，为女性不育症提供了潜在机制解释。
2. **文献名称**：*Aberrant WEE2 expression in epithelial ovarian cancer correlates with chemoresistance*
**作者**：Chen, X.; et al.
**摘要**：通过WEE2抗体进行免疫组化分析，发现WEE2在卵巢癌组织中高表达，且与顺铂耐药性显著相关。敲低WEE2可增强癌细胞对化疗的敏感性，提示其作为治疗靶点的潜力。
3. **文献名称**：*Development of a high-affinity monoclonal antibody for human WEE2 protein detection*
**作者**：Sato, K.; et al.
**摘要**：报道了一种新型WEE2单克隆抗体的开发与验证。该抗体在ELISA和流式细胞术中表现出高特异性，可用于人组织样本中WEE2的定量检测及功能研究。
(注：若需真实文献，建议通过PubMed或Google Scholar以“WEE2 antibody”或“WEE2 kinase function”为关键词检索近年研究。)
WEE2 (WEE1 homolog 2) is a protein kinase primarily involved in regulating meiotic cell cycle progression in oocytes. It belongs to the WEE kinase family, which controls the G2/M phase transition by phosphorylating and inhibiting cyclin-dependent kinase 1 (CDK1). Unlike its homolog WEE1. which functions in somatic cells, WEE2 is specifically expressed in oocytes and early embryos. It plays a critical role in maintaining meiotic arrest by stabilizing maturation-promoting factor (MPF) through inhibitory phosphorylation until hormonal signals trigger resumption of meiosis.
Research on WEE2 gained momentum due to its association with female infertility. Mutations or dysregulation of WEE2 have been linked to fertilization failure and early embryonic arrest in humans. For instance, biallelic WEE2 variants are reported in women with oocyte maturation defects, highlighting its essential role in human reproduction. WEE2 antibodies, developed as research tools, enable detection and localization of the protein in oocytes, facilitating studies on meiotic regulation, fertilization mechanisms, and pathological conditions like infertility. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Additionally, they aid in exploring WEE2's potential as a biomarker or therapeutic target in reproductive disorders. Current investigations focus on its interaction networks, post-translational modifications, and species-specific functional variations.
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