Recombinant Human PSMA5 protein

中文名： 蛋白酶体亚基α5型(PSMA5)重组蛋白
别名： PSMA5;Proteasome subunit alpha type-5

货号: PA1000-2550

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PSMA5
Uniprot No	P28066
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-241aa
氨基酸序列	MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI
预测分子量	31 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PSMA5重组蛋白的3篇参考文献概览：

1. **文献名称**: "Proteasome subunit α5 overexpression promotes proteasome activity in cancer cells and contributes to bortezomib resistance"

**作者**: Chen et al.

**摘要**: 研究通过重组表达人源PSMA5蛋白，分析其在多发性骨髓瘤细胞中的功能，发现PSMA5过表达增强蛋白酶体活性并导致硼替佐米耐药性，揭示了其在肿瘤治疗中的潜在调控机制。

2. **文献名称**: "Structural insights into the assembly and function of the 20S proteasome α5 subunit"

**作者**: Smith et al.

**摘要**: 利用重组PSMA5蛋白进行X射线晶体学研究，解析其三维结构及与其他蛋白酶体亚基的相互作用，阐明其在蛋白酶体组装和底物识别中的关键作用。

3. **文献名称**: "Recombinant expression and purification of the human proteasome subunit PSMA5 for biochemical characterization"

**作者**: Li & Wang

**摘要**: 开发了一种高效的大肠杆菌表达系统，用于重组PSMA5蛋白的制备，优化纯化步骤并验证其生物活性，为后续蛋白酶体复合体的体外重构研究提供基础。

4. **文献名称**: "PSMA5 knockdown impairs protein degradation and induces apoptosis in hepatocellular carcinoma cells"

**作者**: Zhang et al.

**摘要**: 通过RNA干扰和重组PSMA5回补实验，证明PSMA5在肝癌细胞中调控异常蛋白降解通路，其缺失导致内质网应激和细胞凋亡，提示其作为治疗靶点的潜力。

(注：以上文献为示例，实际引用需根据具体研究内容核实。)

背景信息

**Background of PSMA5 Recombinant Protein**

PSMA5 (Proteasome 20S Subunit Alpha 5) is a critical component of the 20S proteasome core complex, a multicatalytic protease responsible for the degradation of ubiquitinated proteins in eukaryotic cells. As part of the α-ring structure of the 20S proteasome, PSMA5 plays a structural role in maintaining the barrel-shaped architecture of the proteasome and regulates substrate entry into the catalytic chamber. This protein is essential for cellular homeostasis, influencing processes such as cell cycle progression, apoptosis, and stress response by controlling protein turnover.

The recombinant PSMA5 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Researchers employ recombinant PSMA5 to study proteasome assembly, substrate recognition mechanisms, and the impact of proteasomal dysfunction in diseases like cancer, neurodegenerative disorders, and autoimmune conditions. Its high purity and activity make it valuable for *in vitro* assays, structural studies (e.g., X-ray crystallography or cryo-EM), and drug discovery efforts targeting proteasome inhibition or modulation.

Studies involving PSMA5 also explore its role as a potential biomarker or therapeutic target, particularly in cancers where proteasome activity is dysregulated. By leveraging recombinant PSMA5. scientists aim to develop selective inhibitors or activators to fine-tune proteasome activity, offering new avenues for treating diseases linked to protein aggregation or aberrant degradation. Overall, PSMA5 recombinant protein serves as a vital tool for unraveling proteasome biology and advancing precision medicine strategies.