| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TIH1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PHLDA3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PHLDA3抗体的三篇参考文献及其摘要内容，涵盖不同研究领域：
1. **文献名称**：*PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors*
**作者**：Kawase T. et al.
**摘要**：该研究揭示了PHLDA3作为胰腺神经内分泌肿瘤(pNET)中的抑癌基因，通过抑制AKT信号通路调控细胞存活。研究中利用PHLDA3抗体进行免疫组化和Western blot分析，证实PHLDA3在肿瘤组织中的低表达与患者不良预后相关。
2. **文献名称**：*PHLDA3 modulates insulin secretion in pancreatic β-cells through regulation of mitochondrial dynamics*
**作者**：Takahashi Y. et al.
**摘要**：本文探讨PHLDA3在胰岛β细胞中通过线粒体分裂调控胰岛素分泌的机制。通过PHLDA3抗体检测蛋白表达水平，发现其缺失导致线粒体过度分裂及胰岛素分泌异常，提示其在糖尿病病理中的潜在作用。
3. **文献名称**：*PHLDA3 promotes apoptosis via interaction with heat shock protein 70 in lung cancer cells*
**作者**：Ohmura S. et al.
**摘要**：研究揭示了PHLDA3在肺癌中通过与HSP70结合诱导细胞凋亡的分子机制。利用PHLDA3抗体进行免疫沉淀和功能抑制实验，证实其促凋亡作用依赖于p53通路，为肺癌治疗提供新靶点。
4. **文献名称**：*Tissue-specific expression of PHLDA3 in mouse models revealed by a novel monoclonal antibody*
**作者**：Yamada K. et al.
**摘要**：该研究开发了一种高特异性PHLDA3单克隆抗体，并应用于小鼠模型的组织分析，发现PHLDA3在胰腺、肾上腺等内分泌器官中高表达，提示其生理功能的组织特异性。
这些文献展示了PHLDA3抗体在肿瘤机制、代谢调控及工具开发中的多样化应用。若需扩展，可进一步检索抗体开发类研究(如第四篇)或特定疾病模型中的验证实验。
The PHLDA3 (Pleckstrin Homology-Like Domain Family A Member 3) antibody is a tool used to detect the PHLDA3 protein, a member of the PH domain-containing protein family involved in apoptosis, cell proliferation, and stress responses. PHLDA3 is encoded by the *PHLDA3* gene, located on human chromosome 1q32.1. and is regulated by the tumor suppressor p53 (TP53). The protein contains a PH-like domain that mediates interactions with lipids or other proteins, influencing intracellular signaling pathways. Notably, PHLDA3 acts as a tumor suppressor by inhibiting the Akt signaling pathway, promoting apoptosis in cancer cells. It is also implicated in metabolic regulation, particularly in pancreatic β-cells, where it interacts with PPARγ to modulate insulin secretion and cell survival under stress.
PHLDA3 expression is tissue-specific, with high levels observed in the pancreas, placenta, and certain brain regions. Dysregulation of PHLDA3 has been linked to cancers (e.g., neuroendocrine tumors, lung adenocarcinoma) and diabetes. The PHLDA3 antibody is widely used in research to study its role in tumor suppression, metabolic diseases, and cellular stress responses via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validated antibodies target specific epitopes, enabling detection of endogenous PHLDA3 (~30-35 kDa) across species, including human, mouse, and rat. Its clinical relevance is emerging, with potential applications as a biomarker or therapeutic target.
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