Recombinant Human MAP1LC3B protein

中文名： 微管相关蛋白 1A/1B 轻链 3B(MAP1LC3B)重组蛋白
别名： FUNDC1;FUN14 domain-containing protein 1

货号: PA1000-1887

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	MAP1LC3B
Uniprot No	Q9GZQ8
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-120aa
氨基酸序列	MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFG
预测分子量	18.2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

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背景信息

MAP1LC3B (microtubule-associated protein 1 light chain 3 beta), also known as LC3B, is a critical autophagy-related protein involved in the formation and maturation of autophagosomes. It is a mammalian homolog of yeast Atg8 and serves as a widely used marker for monitoring autophagy. The native LC3B protein exists in two forms: a cytosolic pro-form (proLC3) that is processed into LC3-I, which is subsequently conjugated to phosphatidylethanolamine (PE) to form LC3-II. LC3-II localizes to autophagosomal membranes, playing essential roles in cargo recognition, membrane expansion, and autophagosome-lysosome fusion.

Recombinant MAP1LC3B protein is produced through genetic engineering techniques, typically expressed in bacterial or mammalian systems. Its purified form retains functional properties, enabling in vitro studies on autophagy mechanisms, protein-protein interactions, and drug screening. Researchers often utilize fluorescently tagged LC3B (e.g., GFP-LC3B) to visualize autophagosome dynamics in live-cell imaging.

This recombinant protein has become indispensable in autophagy research due to its ability to quantify autophagic flux when combined with lysosomal inhibitors. It also facilitates studies linking autophagy to various pathologies, including neurodegenerative diseases, cancer, and infectious diseases. Pharmaceutical applications include screening autophagy-modulating compounds and validating therapeutic targets. Compared to other LC3 isoforms, LC3B shows higher specificity in autophagosome association, making it a preferred choice for experimental models. Its recombinant version maintains consistent activity across applications while avoiding contamination risks associated with native protein purification.