| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ATP5L; ATP5JG |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ATP5MG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ATP5MG抗体的3篇文献摘要示例(注：文献为虚拟示例，实际引用需核实)：
1. **《ATP5MG regulates mitochondrial function in neurodegenerative diseases》**
- **作者**：Smith A, et al.
- **摘要**：研究通过ATP5MG抗体检测发现，ATP5MG蛋白在阿尔茨海默病模型小鼠脑组织中线粒体ATP合酶复合体表达显著降低，提示其可能通过影响能量代谢加剧神经元损伤。
2. **《Role of ATP5MG in cancer cell apoptosis》**
- **作者**：Chen L, et al.
- **摘要**：利用ATP5MG抗体进行免疫印迹和免疫荧光实验，证实ATP5MG在结直肠癌细胞中高表达，且沉默该基因可通过破坏线粒体膜电位诱导癌细胞凋亡。
3. **《ATP5MG deficiency disrupts cardiac energy metabolism》**
- **作者**：Wang Y, et al.
- **摘要**：通过ATP5MG抗体检测心肌细胞线粒体蛋白，发现ATP5MG敲除小鼠心脏ATP合成能力下降，导致心力衰竭，揭示了其在心脏能量稳态中的关键作用。
如需具体文献，建议在PubMed或Google Scholar中检索关键词“ATP5MG antibody”或“ATP synthase membrane subunit g”。
ATP5MG, also known as ATP synthase mitochondrial F0 complex subunit G or ATP5L, is a key component of the mitochondrial ATP synthase complex (Complex V). This subunit is part of the F0 domain, which forms the transmembrane proton channel critical for oxidative phosphorylation. ATP5MG plays a role in maintaining the structural integrity of the ATP synthase and facilitates proton translocation, thereby driving ATP synthesis. Dysregulation of ATP5MG has been linked to mitochondrial dysfunction, impacting cellular energy metabolism and contributing to diseases such as neurodegenerative disorders, cancer, and metabolic syndromes.
ATP5MG antibodies are essential tools for studying the expression, localization, and function of this subunit in various biological contexts. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess ATP synthase assembly, mitochondrial membrane protein profiles, or energy metabolism alterations. Researchers employ these antibodies to investigate ATP5MG's role in cellular responses to stress, apoptosis, or metabolic reprogramming in diseases. Commercially available ATP5MG antibodies are typically raised against specific epitopes of human or mouse origin, with validation in model organisms. Proper controls, such as mitochondrial fractionation or knockout validation, are recommended to ensure antibody specificity due to potential cross-reactivity with other mitochondrial proteins.
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