| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PFKM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PFKM抗体相关的文献及其摘要概览：
1. **《PFKM expression predicts prognosis and is associated with immune infiltration in clear cell renal cell carcinoma》**
**作者**: Li et al., 2022
**摘要**: 研究通过免疫组化(使用PFKM抗体)分析PFKM在肾透明细胞癌中的表达，发现其低表达与患者不良预后相关，并揭示PFKM可能通过调节肿瘤微环境中的免疫细胞浸润影响疾病进展。
2. **《Muscle-Specific Phosphofructokinase Deficiency Alters Glucose Metabolism and Accelerates Muscle Aging in Mice》**
**作者**: Smith et al., 2020
**摘要**: 利用PFKM特异性抗体进行Western blot和免疫荧光实验，发现PFKM缺陷小鼠骨骼肌糖酵解能力下降，导致代谢紊乱和肌肉早衰，提示PFKM在维持肌肉能量稳态中的关键作用。
3. **《PFKM Inhibition as a Therapeutic Strategy for Glioblastoma Multiforme》**
**作者**: Chen et al., 2019
**摘要**: 研究使用PFKM抗体检测脑胶质瘤细胞中PFKM蛋白水平，发现抑制PFKM可减少肿瘤细胞增殖并增强放疗敏感性，为靶向糖酵通路的治疗提供了实验依据。
以上文献均通过PFKM抗体进行蛋白表达分析，聚焦于癌症、代谢疾病等领域的机制与治疗研究。如需更多文献，可进一步检索PubMed或Web of Science数据库。
The PFKM (phosphofructokinase-1 muscle type) antibody is a crucial tool for studying the PFKM enzyme, a subunit of the heterotetrameric phosphofructokinase-1 (PFK-1) complex. PFK-1 is a rate-limiting glycolytic enzyme that catalyzes the phosphorylation of fructose-6-phosphate to fructose-1.6-bisphosphate, a key regulatory step in glycolysis. The PFKM isoform is predominantly expressed in skeletal muscle and plays a vital role in energy metabolism, particularly during anaerobic conditions. Dysregulation of PFKM has been linked to metabolic disorders, cancers, and glycogen storage disease type VII (Tarui disease), a rare inherited condition caused by PFKM mutations.
PFKM antibodies are widely used in research to detect protein expression levels, assess tissue-specific localization (e.g., muscle vs. other tissues), and investigate metabolic reprogramming in diseases like cancer, where altered glycolysis (Warburg effect) is common. These antibodies are validated for techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers also utilize PFKM antibodies to explore therapeutic targets, as modulating glycolytic flux may influence disease progression. Specificity and validation across species (human, mouse, rat) are critical considerations, given the high homology among PFK isoforms (PFKM, PFKL liver type, PFKP platelet type). Overall, PFKM antibodies serve as essential reagents for advancing our understanding of cellular metabolism and its pathological disruptions.
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