| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CNOT10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CNOT10抗体的3篇参考文献示例(内容为模拟，仅供参考)：
1. **"CNOT10 regulates embryonic stem cell differentiation by modulating mRNA decay"**
*Authors: Smith A, et al.*
**摘要**：本研究利用特异性CNOT10抗体，通过免疫沉淀和Western blot分析，证实CNOT10通过CCR4-NOT复合体调控靶mRNA稳定性，影响胚胎干细胞分化的关键基因表达。
2. **"Development and validation of a monoclonal antibody for CNOT10 in human tissues"**
*Authors: Lee JH, et al.*
**摘要**：报道一种高特异性抗人CNOT10单克隆抗体的开发，经质谱验证后应用于免疫组化，发现CNOT10在多种癌症组织中表达异常，提示其潜在肿瘤调控功能。
3. **"CNOT10 interacts with viral RNA to restrict influenza A replication"**
*Authors: Zhang Y, et al.*
**摘要**：通过免疫共沉淀(使用CNOT10抗体)结合RNA-seq，揭示CNOT10直接结合流感病毒RNA并促进其降解，为抗病毒机制研究提供新方向。
*注：上述文献为示例，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"CNOT10 antibody"+"CCR4-NOT complex"+"gene regulation"进一步筛选。*
The CNOT10 antibody is a research tool designed to target the CNOT10 protein, a critical subunit of the CCR4-NOT complex—a conserved eukaryotic multi-protein assembly involved in mRNA decay and post-transcriptional gene regulation. The CCR4-NOT complex plays diverse roles, including deadenylation of mRNA poly(A) tails, translational repression, and chromatin modification, thereby influencing cellular processes like development, stress responses, and apoptosis. CNOT10. specifically, contributes to complex stability and function, interacting with other subunits such as CNOT1. CNOT2/3. and catalytic deadenylases (e.g., CNOT6/6L/7). Studies suggest CNOT10’s involvement in miRNA-mediated gene silencing and maintenance of embryonic stem cell pluripotency.
Antibodies against CNOT10 are widely used in molecular biology to investigate its expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. They aid in exploring CNOT10’s role in diseases, including cancers (e.g., leukemia, colorectal cancer) and neurological disorders, where dysregulated mRNA turnover is implicated. Commercially available CNOT10 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated depletion. Research utilizing these antibodies continues to uncover mechanistic insights into post-transcriptional control networks, offering potential therapeutic targets for conditions linked to CCR4-NOT dysfunction.
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