Rabbit Polyclonal ATP5F1A Antibody

The ATP5F1A antibody targets the alpha subunit of mitochondrial ATP synthase (Complex V), a key enzyme in oxidative phosphorylation. ATP5F1A is integral to the catalytic core of the ATP synthase complex, located in the mitochondrial inner membrane, where it facilitates ATP production by coupling proton translocation to ATP synthesis. This subunit plays a structural and regulatory role, contributing to the enzyme’s stability and activity. Antibodies against ATP5F1A are widely used in research to study mitochondrial function, energy metabolism, and diseases linked to mitochondrial dysfunction, such as cancer, neurodegenerative disorders, and metabolic syndromes. They enable detection of ATP5F1A expression levels, subcellular localization, and post-translational modifications via techniques like Western blotting, immunofluorescence, and immunohistochemistry. In cancer research, ATP5F1A expression patterns may correlate with tumor progression or therapeutic resistance, while in neurodegeneration, altered levels are associated with bioenergetic deficits. Commercial ATP5F1A antibodies are typically validated for specificity across human, mouse, and rat samples. Researchers rely on these tools to explore mitochondrial dynamics, cellular stress responses, and drug mechanisms affecting energy metabolism. Quality control includes verifying reactivity in knockout models or peptide-blocking assays to ensure minimal cross-reactivity with other ATP synthase subunits.