Recombinant Human ING1 protein

ING1 (Inhibitor of Growth 1) is a tumor suppressor protein encoded by the *ING1* gene, initially identified for its role in regulating cell cycle progression, apoptosis, and chromatin remodeling. As a member of the ING family of proteins, it functions as a critical epigenetic modulator by interacting with histone acetyltransferases (HATs) and histone deacetylases (HDACs) to regulate histone acetylation status, thereby influencing gene expression. ING1 exists in two major splice variants, p47ING1a and p33ING1b, which exhibit distinct subcellular localization and biological activities, though both are implicated in stress responses and genomic stability.

Recombinant ING1 protein is engineered to enable detailed biochemical and functional studies. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains key functional domains, including the plant homeodomain (PHD) finger for chromatin binding and the nuclear localization signal (NLS). Its production allows researchers to investigate interactions with partners like p53. proliferating cell nuclear antigen (PCNA), and chromatin-modifying complexes, shedding light on its role in DNA repair, senescence, and tumor suppression.

Dysregulation of ING1 is linked to cancers, neurodegenerative disorders, and aging. Recombinant ING1 facilitates drug discovery, structural analysis, and mechanistic studies, particularly in understanding how its loss or mutation contributes to disease. Its application extends to in vitro assays for screening therapeutic agents targeting epigenetic pathways or restoring ING1 function in malignancies.