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Recombinant Human ING1 protein

  • 中文名: 生长抑制因子1(ING1)重组蛋白
  • 别    名: ING1;Inhibitor of growth protein 1
货号: PA1000-1655
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ING1
Uniprot NoQ9UK53-2
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-279aa
氨基酸序列MGSSHHHHHH SSGLVPRGSH MGS MLSPANG EQ LHLVNYVE DYLDSIESLP FDLQRNVSLM REIDAKYQEI LKELDECYER FSRETDGAQK RR MLHCVQRA LIRSQELGDE KIQIVSQMVE LVENRTRQVD SHVELFEAQQ ELGDTAGNSG KA GADRPKGE AAAQADKPNS KRSRRQRNNE NRENASSNHD HDDGASGTPK EKKAKTSKKK KR SKAKAERE ASPADLPIDP NEPTYCLCNQ VSYGEMIGCD NDECPIEWFH FSCVGLNHKP KG KWYCPKCR GENEKTMDKA LEKSKKERAY NR
预测分子量34 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

1. **"Tumor suppressor ING1: structure and function" by Garkavtsev & Riabowol (1996)**

早期研究阐明ING1基因的克隆及重组蛋白功能,证实其通过调控p53通路抑制肿瘤生长并诱导细胞衰老。

2. **"Crystal structure of the ING1 PHD domain in complex with H3K4me3" by Helbing et al. (2005)**

解析重组ING1蛋白PHD结构域与组蛋白H3K4三甲基化的复合物晶体结构,揭示其表观遗传识别机制及染色质结合模式。

3. **"Recombinant ING1 induces apoptosis in breast cancer cells via caspase activation" by Coles et al. (2003)**

通过体外实验证明纯化重组ING1蛋白通过激活Caspase通路促进乳腺癌细胞凋亡,提示其治疗应用潜力。

4. **"ING1 modulates histone acetylation by recruiting HAT complexes" by Doyon et al. (2004)**

研究重组ING1蛋白与组蛋白乙酰转移酶复合物(如p300)的相互作用,揭示其在染色质重塑及基因转录调控中的桥梁作用。

背景信息

ING1 (Inhibitor of Growth 1) is a tumor suppressor protein encoded by the *ING1* gene, initially identified for its role in regulating cell cycle progression, apoptosis, and chromatin remodeling. As a member of the ING family of proteins, it functions as a critical epigenetic modulator by interacting with histone acetyltransferases (HATs) and histone deacetylases (HDACs) to regulate histone acetylation status, thereby influencing gene expression. ING1 exists in two major splice variants, p47ING1a and p33ING1b, which exhibit distinct subcellular localization and biological activities, though both are implicated in stress responses and genomic stability.

Recombinant ING1 protein is engineered to enable detailed biochemical and functional studies. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains key functional domains, including the plant homeodomain (PHD) finger for chromatin binding and the nuclear localization signal (NLS). Its production allows researchers to investigate interactions with partners like p53. proliferating cell nuclear antigen (PCNA), and chromatin-modifying complexes, shedding light on its role in DNA repair, senescence, and tumor suppression.

Dysregulation of ING1 is linked to cancers, neurodegenerative disorders, and aging. Recombinant ING1 facilitates drug discovery, structural analysis, and mechanistic studies, particularly in understanding how its loss or mutation contributes to disease. Its application extends to in vitro assays for screening therapeutic agents targeting epigenetic pathways or restoring ING1 function in malignancies.

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