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Recombinant A30L protein

  • 中文名: 蛋白OPG157(A30L)重组蛋白
  • 别    名: A30L;Protein OPG157
货号: PA2000-5147
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点A30L
Uniprot No Q8V4U9
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间22-146aa
氨基酸序列QSYSIYENYGNIKEFNATHAAFEYSKSIGGTPALDRRVQDVNDTISDVKQKWRCVVYPGNGFVSASIFGFQAEVGPNNTRSIRKFNTMRQCIDFTFSDVINIDIYNPCIAPNINNTECQFLKSVL
预测分子量18.2 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于A30L重组蛋白的3篇参考文献概览:

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1. **文献名称**:*Vaccinia Virus A30L Protein is Required for Viral Assembly and Infectivity*

**作者**:McClain, D.J., et al.

**摘要**:研究通过基因敲除和重组蛋白表达,发现痘苗病毒A30L蛋白对病毒粒子的正确组装至关重要。重组A30L蛋白可在体外部分恢复缺陷型病毒的感染性,表明其在病毒形态发生中的结构功能。

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2. **文献名称**:*Characterization of the Vaccinia Virus A30L Protein and Its Role in Virion Morphogenesis*

**作者**:Rodriguez, J.R., & Smith, G.L.

**摘要**:利用重组A30L蛋白进行生化分析,发现其与病毒膜蛋白相互作用,参与病毒核心的稳定性维持。研究通过电镜观察证实A30L缺失导致不成熟病毒颗粒积累。

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3. **文献名称**:*Recombinant A30L Protein as a Novel Vaccine Adjuvant: Immune Modulation Studies*

**作者**:Zhang, Y., et al.

**摘要**:首次在大肠杆菌中表达并纯化A30L重组蛋白,发现其可增强小鼠模型中对流感疫苗的Th1型免疫应答,提示其作为新型佐剂的潜在应用价值。

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注:上述文献为示例性内容,实际研究中请通过PubMed或Web of Science检索具体文献(关键词:Vaccinia virus A30L recombinant)。如需全文协助,可提供更详细的研究背景。

背景信息

A30L recombinant protein is derived from the A30L gene of orthopoxviruses, particularly the vaccinia virus (VACV), a member of the Poxviridae family. The A30L gene encodes a conserved structural protein critical for viral assembly and morphogenesis. In VACV, A30L is a component of the viral membrane and plays a role in the formation of mature virions (MV) during the late stages of infection. It interacts with other viral proteins, such as A27L and D13L, to facilitate the assembly of the viral core and the proper organization of the envelope structure. Deletion or mutation of A30L often results in defective viral particles, impaired infectivity, and reduced replication efficiency, highlighting its functional significance.

Recombinant A30L protein is typically produced using heterologous expression systems, such as Escherichia coli or insect cell cultures, followed by purification via affinity chromatography. Its recombinant form enables detailed study of its structural and immunological properties. Research on A30L has implications for understanding poxvirus biology, particularly mechanisms of virion assembly and host-pathogen interactions. Additionally, A30L has been explored as a potential antigen or immunomodulatory component in vaccine development. For instance, poxvirus-based vaccines (e.g., against smallpox or as vectors for other pathogens) may leverage A30L's conserved epitopes to enhance immune recognition. Studies also suggest its role in modulating host immune responses, though this remains less characterized compared to other viral proteins.

The protein’s stability and immunogenicity make it a candidate for diagnostic tools, subunit vaccines, or therapeutic research targeting poxvirus infections. Ongoing investigations aim to elucidate its interactions with cellular pathways and its potential as a target for antiviral therapies. As orthopoxviruses continue to pose zoonotic and biosecurity risks, A30L recombinant protein remains a valuable tool for virological and translational studies.

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