Recombinant Human GORASP2 protein

中文名： 高尔基体重组堆积蛋白2(GORASP2)重组蛋白
别名： GORASP2;GOLPH6;Golgi reassembly-stacking protein 2

货号: PA1000-1304

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	GORASP2
Uniprot No	Q9H8Y8
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-452aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSMGSSQSV EIPGGGTEGY HVLRVQENSP GHRAGLEPFF DFIVSINGSR LNKDNDTLKD LLKANVEKPV KMLIYSSKTL ELRETSVTPS NLWGGQGLLG VSIRFCSFDG ANENVWHVLE VESNSPAALA GLRPHSDYII GADTVMNESE DLFSLIETHE AKPLKLYVYN TDTDNCREVI ITPNSAWGGE GSLGCGIGYG YLHRIPTRPF EEGKKISLPG QMAGTPITPL KDGFTEVQLS SVNPPSLSPP GTTGIEQSLT GLSISSTPPA VSSVLSTGVP TVPLLPPQVN QSLTSVPPMN PATTLPGLMP LPAGLPNLPN LNLNLPAPHI MPGVGLPELV NPGLPPLPSM PPRNLPGIAP LPLPSEFLPS FPLVPESSSA ASSGELLSSL PPTSNAPSDP ATTTAKADAA SSLTVDVTPP TAKAPTTVED RVGDSTPVSE KPVSAAVDAN ASESP
预测分子量	50 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于GORASP2重组蛋白的模拟参考文献示例(仅供格式参考，具体文献需通过学术数据库验证)：

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1. **"Recombinant GORASP2 Facilitates Golgi Stacking in vitro"**

*Author: Chen et al. (2018)*

摘要：研究利用重组GORASP2蛋白在体外重构高尔基体 stacking 的过程，证明其通过寡聚化调控膜结构组装，为高尔基体形态维持机制提供证据。

2. **"GORASP2 Knockdown Impairs Protein Secretion via Disrupting ER-Golgi Transport"**

*Author: Smith et al. (2020)*

摘要：通过表达重组GORASP2挽救基因敲除细胞模型，揭示其在内质网-高尔基体囊泡运输中的关键作用，并影响分泌蛋白的成熟。

3. **"Crystal Structure of the GRASP Domain in GORASP2 Reveals Dimerization Interface"**

*Author: Tanaka et al. (2019)*

摘要：解析重组GORASP2的GRASP结构域晶体结构，阐明其二聚化机制及与Rab GTPases的互作位点，为靶向药物设计提供结构基础。

4. **"GORASP2 Promotes Cancer Metastasis via MMP9 Secretion Regulation"**

*Author: Li & Wang (2021)*

摘要：在肿瘤细胞中过表达重组GORASP2.发现其通过增强MMP9的分泌促进细胞侵袭，提示其作为癌症治疗潜在靶点。

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**提示**：以上为模拟文献，实际研究请通过PubMed、Google Scholar等平台以“GORASP2”(或“GRASP2”)及“recombinant protein”为关键词检索最新成果。

背景信息

GORASP2 (Golgi ReAssembly Stacking Protein 2), also known as GRASP55. is a ubiquitously expressed protein primarily localized to the Golgi apparatus. It plays a critical role in maintaining Golgi structure and regulating vesicular trafficking. As a member of the GRASP family, it contains N-terminal PDZ domains and a C-terminal SPCR motif, enabling its function in membrane tethering and stacking of Golgi cisternae. Unlike its paralog GRASP65 (GORASP1), which operates in the cis-Golgi, GRASP55 predominantly acts in the medial/trans-Golgi compartments.

Recombinant GORASP2 proteins are engineered to study Golgi dynamics, particularly in contexts like mitosis, cellular stress, or pathological conditions. Researchers produce these proteins using heterologous expression systems (e.g., E. coli, mammalian cells) with tags (e.g., His, GFP) for purification and tracking. Studies using recombinant GORASP2 have revealed its involvement in unconventional protein secretion, autophagy, and ER-Golgi communication. It also participates in O-glycosylation regulation and collagen export, linking it to developmental disorders and connective tissue diseases.

Notably, GORASP2 dysregulation is implicated in cancer progression and neurodegenerative diseases. Its phosphorylation status, modulated by kinases like CDK1 and PLK1 during cell cycle transitions, governs Golgi disassembly-reassembly cycles. Recombinant forms enable structural studies (e.g., crystallography) to map interaction interfaces and screen small-molecule modulators. Current research leverages GORASP2 recombinant proteins to dissect Golgi-related mechanisms in cellular homeostasis and develop therapeutic strategies targeting secretory pathway defects.