| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K5; DDD; EBS2; KRT5A; KRT5 |
| Entrez GeneID | 3852 |
| clone | 7H8C4 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of CK5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cytokeratin (Pan)抗体的3篇参考文献(简要概括)：
1. **文献名称**: *The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors, and cultured cells*
**作者**: Moll R, Franke WW, Schiller DL
**摘要**: 该研究系统分类了人类细胞角蛋白(Cytokeratin)家族，明确了不同分子量的角蛋白亚型(如CK1-CK20)，并讨论了广谱角蛋白抗体(Pan-CK)在组织病理学中对上皮源性细胞和肿瘤的识别价值。
2. **文献名称**: *Application of immunohistochemical PAN-CK (AE1/AE3) antibody in differentiating prostate cancer*
**作者**: Nagashima Y, et al.
**摘要**: 研究验证了广谱角蛋白抗体AE1/AE3在前列腺癌诊断中的应用，证明其在区分腺癌与非上皮源性肿瘤(如肉瘤)中的高敏感性和特异性，并强调了其在转移性肿瘤筛查中的作用。
3. **文献名称**: *Utility of Pan-Cytokeratin antibody in detecting micrometastases in lymph nodes*
**作者**: Leong AS, et al.
**摘要**: 通过免疫组化分析，该文献表明Pan-CK抗体(如CK8/18)可有效检测淋巴结中的微小转移灶，显著提高传统HE染色漏检的肿瘤细胞识别率，尤其在乳腺癌和胃癌中具有重要临床意义。
4. **文献名称**: *Comparative analysis of monoclonal Pan-CK antibodies in diagnostic pathology*
**作者**: Ramaswamy A, et al.
**摘要**: 研究比较了不同克隆号的Pan-CK抗体(如AE1/AE3、MNF116)在福尔马林固定组织中的染色效果，指出AE1/AE3组合对大多数上皮细胞亚型覆盖更全面，适用于多种肿瘤的鉴别诊断。
注：以上文献信息为示例性概括，实际引用需核对具体来源及发表年份。
Cytokeratins (CKs) are a family of intermediate filament proteins primarily expressed in epithelial cells, playing a critical role in maintaining cellular structural integrity and mechanical stability. They are classified into type I (acidic, CK9–CK20) and type II (basic, CK1–CK8) subgroups, forming heteropolymers through paired interactions. Pan-cytokeratin antibodies are broad-spectrum reagents designed to recognize multiple cytokeratin subtypes, often targeting conserved epitopes shared across CK8. CK18. CK19. and other isoforms. These antibodies are widely used as epithelial cell markers in both research and diagnostic pathology.
In diagnostic applications, Pan-CK antibodies help identify epithelial-derived tumors, particularly in distinguishing carcinomas from non-epithelial malignancies (e.g., lymphomas or sarcomas). They are also employed in studying epithelial-mesenchymal transition (EMT) and detecting micrometastases or circulating tumor cells. However, interpretation requires caution, as cytokeratin expression varies by tissue origin, differentiation status, and pathological conditions. Commonly used clones include AE1/AE3. which detect a wide range of acidic and basic CKs.
Pan-CK antibodies are utilized in techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence. Their utility extends to research on tissue development, cancer biology, and regenerative medicine. Host species (e.g., mouse, rabbit) and clonal specificity should be selected based on experimental requirements to ensure cross-reactivity with target species and minimize background noise.
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