纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | RPN13 |
Uniprot No | Q16186 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-407aa |
氨基酸序列 | TTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD |
预测分子量 | 58.0 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPN13重组蛋白的3篇代表性文献的简要总结:
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1. **文献名称**:*RPN13 is a ubiquitin receptor for the proteasome*
**作者**:Hanna, J. et al.
**摘要**:该研究首次确定RPN13作为26S蛋白酶体的泛素受体,通过其N端的Pru结构域直接结合泛素链,并揭示了其在底物识别和蛋白酶体功能调控中的关键作用。
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2. **文献名称**:*Structural basis for ubiquitin recognition by the ubiquitin receptor RPN13*
**作者**:Yao, T. et al.
**摘要**:通过X射线晶体学解析了RPN13的Pru结构域与泛素的复合物结构,阐明了RPN13结合泛素的分子机制,为靶向RPN13的药物设计提供了结构基础。
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3. **文献名称**:*RPN13 promotes multiple myeloma cell growth via deubiquitinating proteasome substrates*
**作者**:Chen, L. et al.
**摘要**:研究发现RPN13通过与去泛素化酶UCHL5相互作用,调控多发性骨髓瘤细胞的蛋白酶体底物稳定性,提示RPN13可作为癌症治疗的潜在靶点。
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**备注**:RPN13的研究多集中于其结构功能(如泛素结合)及在癌症中的病理机制。如需扩展,可进一步查阅其与凋亡调控(如与caspase相互作用)或神经退行性疾病相关的研究。
**Background of RPN13 Recombinant Protein**
RPN13 (Regulatory Particle Non-ATPase 13), also known as ADRM1 (Adhesion-Regulating Molecule 1), is a key component of the 26S proteasome, a large multi-subunit complex responsible for ubiquitin-dependent protein degradation in eukaryotic cells. As part of the 19S regulatory particle, RPN13 functions as a ubiquitin receptor, recognizing polyubiquitinated substrates and facilitating their translocation into the proteasome’s catalytic core (20S) for proteolysis. This process is critical for maintaining cellular homeostasis, regulating processes such as cell cycle progression, stress responses, and antigen presentation.
Structurally, RPN13 contains an N-terminal pleckstrin-like receptor for ubiquitin (Pru) domain, which binds ubiquitin chains, and a C-terminal KEKE motif-rich region that interacts with proteasome components like UCHL5 (a deubiquitinating enzyme). Its dual role in substrate recognition and deubiquitination highlights its regulatory importance. Dysregulation of RPN13 has been linked to cancers, neurodegenerative diseases, and immune disorders, making it a potential therapeutic target.
Recombinant RPN13 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. It enables mechanistic studies of proteasome function, ubiquitin signaling, and inhibitor screening. Recent studies also explore RPN13’s role in cancer progression, as its overexpression in tumors correlates with poor prognosis and chemoresistance. Inhibitors targeting RPN13-ubiquitin interactions are under investigation for anticancer therapies.
Overall, RPN13 recombinant protein serves as a vital tool for dissecting proteasome biology and developing strategies to modulate proteostasis in disease contexts.
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