Recombinant Human C1orf194 protein

C1orf194 (Chromosome 1 Open Reading Frame 194) is a poorly characterized human gene located on the short arm of chromosome 1 (1p36.11). Its biological function remains largely undefined, though genomic studies suggest it encodes a conserved protein across vertebrates, hinting at potential roles in fundamental cellular processes. The gene spans approximately 15 kb and produces multiple transcript variants through alternative splicing, though the full protein-coding potential requires further validation.

Recombinant C1orf194 protein refers to the artificially expressed and purified form of this protein, typically generated using bacterial (e.g., E. coli) or eukaryotic expression systems. This engineered approach enables structural and functional studies that are challenging with endogenous protein due to low natural expression levels. Bioinformatic analyses predict C1orf194 contains several disordered regions and potential phosphorylation sites, suggesting possible involvement in signaling pathways or protein-protein interactions. However, experimental evidence remains scarce.

Current research focuses on establishing its subcellular localization, interactome, and potential associations with disease. Preliminary data from high-throughput studies tentatively link C1orf194 to DNA damage response mechanisms and cell cycle regulation. Its recombinant form serves as crucial material for antibody production, enzymatic assays, and structural characterization efforts. Challenges persist in elucidating its precise molecular mechanisms, with ongoing studies aiming to clarify whether it functions as an enzyme, scaffold protein, or regulatory factor. The development of recombinant C1orf194 protein represents a critical step toward decoding its biological significance and potential biomedical relevance.