纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CKMT2 |
Uniprot No | P17540 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 40-419aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK |
预测分子量 | 46 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKMT2重组蛋白的3篇参考文献示例(注:内容为模拟生成,非真实文献):
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1. **文献名称**:*Structural and functional characterization of recombinant human CKMT2*
**作者**:Smith A, et al.
**摘要**:本研究成功在大肠杆菌中表达了重组人源CKMT2蛋白,并通过X射线晶体学解析了其三维结构。功能实验表明,重组CKMT2具有ATP-肌酸磷酸转移酶活性,并依赖线粒体膜脂质环境维持稳定性,为能量代谢研究提供了分子基础。
2. **文献名称**:*CKMT2 overexpression rescues mitochondrial dysfunction in cardiac cells*
**作者**:Chen L, et al.
**摘要**:通过哺乳动物细胞系统表达重组CKMT2蛋白,发现其能显著改善心肌细胞线粒体ATP合成效率,并缓解缺氧诱导的细胞凋亡,提示CKMT2在心脏疾病治疗中的潜在应用价值。
3. **文献名称**:*Role of CKMT2 in cancer metabolism: Insights from recombinant protein studies*
**作者**:Wang Y, et al.
**摘要**:利用HEK293细胞表达纯化的CKMT2重组蛋白,结合肿瘤细胞模型分析,发现CKMT2通过调控磷酸肌酸穿梭促进肿瘤细胞增殖,为靶向CKMT2的癌症治疗策略提供了实验依据。
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(注:如需真实文献,建议通过PubMed或Google Scholar检索“CKMT2 recombinant protein”获取。)
**Background of CKMT2 Recombinant Protein**
Creatine kinase mitochondrial 2 (CKMT2) is a nuclear-encoded enzyme localized in the mitochondrial intermembrane space, belonging to the creatine kinase (CK) family. This enzyme plays a critical role in cellular energy metabolism by catalyzing the reversible transfer of a high-energy phosphate group from ATP to creatine, generating phosphocreatine (PCr) and ADP. PCr serves as an energy reservoir, rapidly regenerating ATP in tissues with high and fluctuating energy demands, such as skeletal muscle, heart, and brain.
CKMT2 is specifically expressed in tissues reliant on oxidative phosphorylation, where it facilitates the spatial buffering of ATP between mitochondria and cytosolic ATP-consuming processes. Its function is closely linked to mitochondrial respiration, making it essential for maintaining energy homeostasis, particularly during stress or increased metabolic activity. Dysregulation of CKMT2 has been implicated in pathologies like cardiomyopathy, neurodegenerative disorders, and cancer, where mitochondrial dysfunction contributes to disease progression.
Recombinant CKMT2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable detailed biochemical and structural studies. Purified recombinant CKMT2 retains enzymatic activity, allowing researchers to investigate its kinetics, substrate specificity, and interactions with regulatory molecules. It is also utilized to develop assays for screening modulators of CKMT2 activity, which may have therapeutic potential. Furthermore, recombinant CKMT2 serves as a tool to study its role in mitochondrial dynamics, apoptosis, and metabolic adaptation under varying physiological or pathological conditions.
Overall, CKMT2 recombinant protein is a vital resource for advancing our understanding of mitochondrial energy metabolism and its implications in health and disease.
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