| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FLJ00204; Plenty of SH3s 2; plenty of SH3s-2; POSH2; SH3 domain containing ring finger 3 |
| Entrez GeneID | 344558; |
| WB Predicted band size | 93kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from C-terminal of human SH3RF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SH3RF3抗体的参考文献摘要(虚构示例，供参考)：
1. **文献名称**：*SH3RF3 regulates mitochondrial apoptosis in hepatocellular carcinoma through JNK signaling*
**作者**：Li X, Wang Y, et al.
**摘要**：该研究利用SH3RF3特异性抗体进行Western blot和免疫组化分析，发现SH3RF3通过调控JNK信号通路抑制肝癌细胞线粒体凋亡，其高表达与患者预后不良相关。
2. **文献名称**：*SH3RF3 interacts with Parkin and modulates mitophagy in neurodegenerative models*
**作者**：Smith J, Brown K, et al.
**摘要**：通过免疫共沉淀(使用SH3RF3抗体)和质谱分析，揭示SH3RF3与Parkin蛋白相互作用，负调控线粒体自噬过程，可能与帕金森病发病机制相关。
3. **文献名称**：*Development of a novel monoclonal antibody against SH3RF3 for diagnostic applications in breast cancer*
**作者**：Zhang R, Liu T, et al.
**摘要**：研究团队开发了一种高特异性抗SH3RF3单克隆抗体，验证其在乳腺癌组织中的灵敏度和特异性，证明SH3RF3可作为潜在生物标志物用于临床诊断。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed。)
SH3RF3 (SH3 domain-containing RING finger protein 3), also known as POSH2 or PSD60. is an E3 ubiquitin ligase involved in regulating cellular processes such as apoptosis, protein trafficking, and signal transduction. It contains multiple SH3 domains that mediate protein-protein interactions and a RING finger domain responsible for its ubiquitin ligase activity. SH3RF3 is implicated in modulating c-Jun N-terminal kinase (JNK) signaling pathways by interacting with scaffold proteins like POSH (Plenty of SH3 domains), thereby influencing stress response, neuronal development, and immune regulation. Dysregulation of SH3RF3 has been linked to cancers, neurodegenerative disorders, and autoimmune diseases.
SH3RF3 antibodies are essential tools for studying its expression, localization, and function in these contexts. They enable detection of endogenous SH3RF3 in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies targeting distinct domains (e.g., SH3 or RING) help dissect its molecular interactions and enzymatic activity. Research using SH3RF3 antibodies has revealed its role in ubiquitinating substrates like MAPK kinases, affecting JNK pathway activation, and its involvement in pathological protein aggregation in conditions like Alzheimer’s disease. Validated antibodies are critical for exploring SH3RF3’s therapeutic potential as a biomarker or drug target.
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