| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SH2 domain-containing protein 1B EWS/FLI1-activated transcript 2; EAT-2 SH2D1B; |
| Entrez GeneID | 117157; |
| WB Predicted band size | 15kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human SH2D1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SH2D1B抗体的3篇参考文献示例(内容基于领域内相关研究概括，非真实文献)：
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1. **文献名称**：*SH2D1B regulates NK cell signaling and cytotoxicity in tumor immunity*
**作者**：Smith A, et al.
**摘要**：本研究通过Western blot和流式细胞术分析SH2D1B在NK细胞中的表达，利用特异性抗体证实其与SLAM家族受体的相互作用，揭示SH2D1B缺失导致NK细胞活化信号减弱，抑制肿瘤杀伤能力。
2. **文献名称**：*EAT-2 (SH2D1B) mutations in immunodeficiency disorders: functional characterization*
**作者**：Chen L, et al.
**摘要**：通过免疫沉淀和质谱技术，研究者使用抗SH2D1B抗体分析患者淋巴细胞样本，发现SH2D1B突变体丧失结合SAP蛋白的能力，导致免疫突触形成缺陷，与严重联合免疫缺陷相关。
3. **文献名称**：*SH2D1B antibody-based profiling in T cell lymphoma pathogenesis*
**作者**：Wang Y, et al.
**摘要**：利用SH2D1B单克隆抗体进行免疫组化分析，发现T细胞淋巴瘤中SH2D1B表达显著上调，其过度激活ERK信号通路促进细胞增殖，提示其作为潜在治疗靶点。
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**备注**：以上文献为模拟概括，实际引用请通过PubMed或Google Scholar检索关键词(如SH2D1B/EAT-2、antibody、immune signaling)。真实研究多聚焦于SH2D1B在免疫细胞(如NK/T细胞)中的信号调控及疾病机制。
SH2D1B, also known as EAT-2 (EWS-activated transcript 2), is a signaling adaptor protein encoded by the *SH2D1B* gene. It belongs to the SLAM (Signaling Lymphocytic Activation Molecule)-associated protein (SAP) family, which regulates immune cell activation and communication. Structurally, SH2D1B contains a single SH2 domain that facilitates interactions with phosphorylated tyrosine residues on SLAM family receptors, such as CD150. CD244. and CD84. expressed on T cells, natural killer (NK) cells, and other immune cells. These interactions modulate downstream signaling pathways, including cytokine production (e.g., IFN-γ) and cytotoxic activity, critical for antiviral and antitumor responses.
SH2D1B antibodies are valuable tools for studying immune regulation, particularly in contexts like X-linked lymphoproliferative disease (XLP) and immune checkpoint research. Mutations in *SH2D1B* or related genes (e.g., *SH2D1A/SAP*) can disrupt SLAM receptor signaling, leading to immune dysregulation. Antibodies targeting SH2D1B enable detection of protein expression via techniques like Western blotting, immunohistochemistry, and flow cytometry. They also aid in exploring SH2D1B’s role in NK cell-mediated cytotoxicity, T cell exhaustion, and cancer immunotherapy resistance. Commercially available SH2D1B antibodies vary in specificity, requiring validation for cross-reactivity with homologous proteins like SAP (SH2D1A). Research using these antibodies contributes to understanding immune pathologies and developing therapies targeting SLAM-associated pathways.
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