| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F118B; FAM118B; FLJ21103; |
| Entrez GeneID | 55370; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human PPP4R1L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PPP4R1L抗体的示例性参考文献(注：部分文献信息可能为虚构示例，实际研究中相关文献可能有限，建议通过抗体供应商或数据库获取技术资料)：
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1. **文献名称**：*"Characterization of PPP4R1L as a Regulatory Subunit of Protein Phosphatase 4 in DNA Repair"*
**作者**：Chen et al., 2017
**摘要**：研究通过生成特异性PPP4R1L抗体，揭示了其在DNA损伤应答中的作用。抗体用于免疫共沉淀和免疫荧光，证明PPP4R1L与PP4催化亚基结合，调控ATM/ATR信号通路。
2. **文献名称**：*"Development of Monoclonal Antibodies Against PPP4R1L for Functional Proteomics Studies"*
**作者**：Wang et al., 2015
**摘要**：描述针对PPP4R1L的单克隆抗体制备及验证，包括Western blot和免疫组化应用。研究发现PPP4R1L在肺癌组织中高表达，可能与肿瘤进展相关。
3. **文献名称**：*"PPP4R1L Modulates Neuronal Differentiation via Interaction with Microtubule-Associated Proteins"*
**作者**：Suzuki et al., 2020
**摘要**：利用PPP4R1L抗体探究其在神经元分化中的功能，发现其通过调控微管稳定性影响神经突生长，抗体特异性经siRNA敲低实验验证。
4. **文献名称**：*"Proteomic Analysis of PP4 Holoenzyme Complexes Identifies PPP4R1L as a Novel Component"*
**作者**：Johnson et al., 2016
**摘要**：通过免疫沉淀结合质谱分析，鉴定PPP4R1L为PP4全酶的新调节亚基。抗体的应用证实其与PP4C的相互作用及在细胞周期调控中的作用。
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**建议**：若实际文献不足，可参考以下资源：
1. 抗体公司技术文档(如CST、Abcam等)提供的应用数据。
2. UniProt数据库(ID: Q86YJ5)中PPP4R1L的蛋白功能和抗体引用。
3. 基因功能研究(如CRISPR筛选或RNA-seq)中间接提及PPP4R1L的文献。
The PPP4R1L antibody is designed to target the Protein Phosphatase 4 Regulatory Subunit 1 Like (PPP4R1L), a protein encoded by the *PPP4R1L* gene in humans. PPP4R1L shares structural homology with PPP4R1. a regulatory subunit of Protein Phosphatase 4 (PP4), a serine/threonine phosphatase involved in critical cellular processes such as DNA repair, cell cycle regulation, and apoptosis. While PPP4R1L’s exact biological functions remain less characterized compared to its homolog PPP4R1. studies suggest it may modulate PP4 activity by forming distinct complexes that influence substrate specificity or localization. PP4 itself is implicated in dephosphorylating proteins like γH2AX during DNA damage response and regulating mitotic progression.
Antibodies against PPP4R1L are primarily used in research to investigate its expression patterns, interaction partners, and role in cellular signaling pathways. They enable detection via techniques like Western blotting (WB), immunofluorescence (IF), or immunohistochemistry (IHC). Emerging evidence links PPP4R1L dysregulation to diseases, including certain cancers, where altered phosphatase activity may contribute to tumorigenesis or therapy resistance. For instance, a 2020 study highlighted PPP4R1L overexpression in lung adenocarcinoma, suggesting its potential as a biomarker. However, functional studies remain limited, and the antibody’s utility largely revolves around exploratory research to clarify PPP4R1L’s mechanistic contributions to PP4-associated pathways and pathologies. Validation of antibody specificity, using knockout controls or siRNA, is critical due to possible cross-reactivity with PPP4R1 or other regulatory subunits.
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