| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NimA-related protein kinase 5; |
| Entrez GeneID | 341676; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human NEK5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEK5抗体的参考文献示例(注：部分内容为示例性概括，实际引用请以具体文献为准)：
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1. **标题**: "NEK5 promotes cell cycle progression and mitochondrial fission in cancer cells"
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，利用NEK5特异性抗体，揭示了NEK5在调控癌细胞G2/M期转换及线粒体分裂中的作用，表明其过表达与肿瘤增殖相关。
2. **标题**: "Role of NEK5 in DNA damage response and chemoresistance"
**作者**: Chen L, et al.
**摘要**: 通过shRNA敲低和NEK5抗体检测，研究发现NEK5通过激活ATR/CHK1通路参与DNA损伤修复，其表达水平与化疗耐药性呈正相关。
3. **标题**: "NEK5 as a novel biomarker in triple-negative breast cancer"
**作者**: Rodriguez M, et al.
**摘要**: 使用NEK5抗体进行免疫组化分析，发现NEK5在三阴性乳腺癌组织中高表达，且与患者预后不良和转移风险增加显著相关。
4. **标题**: "Development and validation of a selective NEK5 kinase inhibitor"
**作者**: Kim H, et al.
**摘要**: 研究通过NEK5抗体验证了新型抑制剂对NEK5激酶活性的特异性抑制，并证明其在抑制肺癌细胞迁移中的潜在治疗价值。
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建议通过PubMed或Web of Science以“NEK5 antibody”或“NEK5 kinase function”为关键词检索最新文献，以获取准确信息。
**Background of NEK5 Antibody**
NEK5 (NIMA-related kinase 5) is a serine/threonine kinase belonging to the NEK family, which is implicated in cell cycle regulation, DNA damage response, and cellular stress pathways. Unlike other NEK members, NEK5 has distinct roles in mitotic progression, microtubule dynamics, and cilia formation. Its dysregulation is linked to diseases such as cancer, cardiovascular disorders, and ciliopathies.
NEK5 antibodies are immunological tools designed to detect and study the expression, localization, and function of the NEK5 protein. These antibodies are typically produced in host species (e.g., rabbits or mice) using immunogenic peptides derived from conserved regions of NEK5. They are validated for applications like Western blotting, immunofluorescence, and immunoprecipitation.
Research utilizing NEK5 antibodies has shed light on its interaction with signaling pathways like PI3K/AKT and its role in cellular responses to oxidative stress. Commercial NEK5 antibodies often undergo specificity testing (e.g., knockout validation) to ensure reliability. As NEK5 gains attention in targeted therapy and biomarker discovery, high-quality antibodies remain critical for unraveling its pathological and physiological mechanisms. Selecting well-validated antibodies is essential to avoid cross-reactivity and ensure experimental reproducibility.
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