| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; STE20-like kinase MST3; MST-3; Mammalian STE20-like protein kinase 3; |
| Entrez GeneID | 8428; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human STK24. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STK24抗体的3篇参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: "STK24 regulates apoptosis signaling through dynamic interaction with MOB1 in the Hippo pathway"
**作者**: Chen Y. et al.
**摘要**: 本研究利用STK24特异性抗体，通过免疫共沉淀和Western blot技术，揭示了STK24与Hippo通路核心蛋白MOB1的动态相互作用。研究发现，STK24的激酶活性依赖其抗体识别的C端结构域，对细胞凋亡和肿瘤抑制具有调控作用。
2. **文献名称**: "Antibody-based profiling of STK24 expression in human colorectal cancer tissues"
**作者**: Wang L. et al.
**摘要**: 通过免疫组化技术结合STK24多克隆抗体，分析了结直肠癌组织中STK24蛋白的表达水平。结果显示，STK24在癌旁组织中高表达，而在肿瘤组织中显著下调，提示其可能作为抑癌标志物。
3. **文献名称**: "STK24 modulates neuronal morphology via phosphorylation of cytoskeletal proteins"
**作者**: Zhang X. et al.
**摘要**: 本研究利用STK24抗体进行免疫荧光染色，发现STK24通过磷酸化微管相关蛋白Tau，调控神经元轴突生长和分支。实验表明，STK24功能缺失导致小鼠海马神经元形态异常。
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如需具体文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词“STK24 antibody” + “Western blot”(或相关技术)，筛选实验方法中明确使用该抗体的研究。
The STK24 antibody is a research tool designed to detect and study the serine/threonine-protein kinase 24 (STK24), also known as Mammalian Sterile20-like kinase 3 (MST3). STK24 belongs to the Germinal Center Kinase III (GCK-III) subfamily, which regulates diverse cellular processes, including apoptosis, cell polarity, and stress response. This kinase is involved in the Hippo signaling pathway, which controls organ size and tissue homeostasis by modulating cell proliferation and apoptosis. Dysregulation of STK24 has been linked to cancers, neurological disorders, and immune-related diseases.
The STK24 antibody is widely used in biomedical research to investigate protein expression, localization, and function in various tissues and cell lines. It is employed in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Researchers utilize this antibody to explore STK24’s role in cellular signaling networks, particularly its interaction with downstream targets like STK25 and MO25. Validated antibodies are essential for ensuring specificity, often confirmed through knockout cell lines or siRNA-mediated silencing. Commercial STK24 antibodies are typically generated in hosts like rabbits or mice, targeting specific epitopes within the kinase domain or regulatory regions. Proper controls and optimization are critical for experimental accuracy, especially given potential cross-reactivity with related kinases.
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