| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Septin-14; SEPT14; |
| Entrez GeneID | 346288; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from C-terminal of human SEPT14. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于SEPT14抗体的代表性文献摘要(基于模拟数据整理)：
1. **文献名称**: *SEPT14 is required for the integrity of the sperm tail and male fertility in mice*
**作者**: Lin, Y.H., et al.
**摘要**: 本研究利用SEPT14特异性抗体发现，SEPT14在小鼠精子尾部形成中起关键作用。抗体免疫荧光显示SEPT14定位于精子尾部中段和主段，基因敲除导致精子形态异常及雄性不育。
2. **文献名称**: *Expression and functional analysis of SEPT14 in human testis*
**作者**: Kuo, Y.C., et al.
**摘要**: 通过Western blot和免疫组化分析，SEPT14抗体证实其在人类睾丸生精细胞中高表达，尤其在精子细胞阶段。研究提示SEPT14可能参与精子顶体形成和细胞分裂调控。
3. **文献名称**: *SEPT14 mutations lead to teratozoospermia and male infertility*
**作者**: Ji, Z., et al.
**摘要**: 该研究使用SEPT14抗体检测临床样本，发现SEPT14突变患者精子中蛋白表达显著降低，导致精子头部畸形和鞭毛结构缺陷，提示其作为男性不育的潜在生物标志物。
4. **文献名称**: *Development of a monoclonal SEPT14 antibody for detecting sperm defects*
**作者**: Hall, J.E., et al.
**摘要**: 报道了一种高特异性SEPT14单克隆抗体的开发，用于临床精液分析。该抗体可有效识别精子尾部结构异常，辅助诊断少弱畸精子症。
(注：以上文献为示例性质，实际引用需以真实发表的论文为准。)
The SEPT14 antibody is a research tool designed to detect and study the SEPT14 protein, a member of the septin family involved in diverse cellular processes. Septins are GTP-binding proteins that contribute to cytoskeletal organization, cell division, vesicle trafficking, and membrane remodeling. SEPT14. specifically, is expressed in tissues such as the testis, brain, and immune cells, with emerging roles in spermatogenesis, neuronal development, and immune regulation. Studies highlight its importance in male fertility, where it participates in sperm head shaping and tail formation during spermiogenesis. In the central nervous system, SEPT14 is implicated in neuronal differentiation and synaptic plasticity.
The SEPT14 antibody enables researchers to investigate the protein's expression patterns, subcellular localization, and interactions via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Dysregulation of SEPT14 has been linked to pathological conditions, including teratozoospermia, neurodegenerative disorders, and certain cancers. For example, reduced SEPT14 levels correlate with abnormal sperm morphology, while aberrant expression is observed in gliomas and leukemia.
By facilitating the study of SEPT14's molecular mechanisms, this antibody aids in unraveling its physiological and pathological roles, potentially informing therapeutic strategies for infertility, neurological diseases, or malignancies. Commercial SEPT14 antibodies are typically validated for specificity using knockout controls or siRNA-based depletion to ensure reliable experimental outcomes.
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